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Scepter 2.0 analysis

Manufactured by Merck Group
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The Scepter 2.0 is a handheld cell counter designed for quick and accurate cell counting. It utilizes impedance-based technology to determine the size and concentration of cells in a sample. The device is capable of analyzing cell samples within a range of 0.4 to 20 micrometers in diameter.

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Scepter 2.0 (27 citations)

2 protocols using scepter 2.0 analysis

1

Cell Growth Kinetics Monitoring

Cited 1 time
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A total of 6 × 104 HaCaT and CaCo-2 cells were seeded in 12-well plate, while HEK293T were seeded on pre-treated 12-well; cells were serum starved for 24 h; after starvation, recombinant or CCM-YB-1 were added at different concentrations. Every 24 h cells were gently rinsed with 1× PBS, trypsinized and counted. The count was confirmed by Scepter 2.0 analysis (Millipore, Burlington, MI, USA) as previously described [36 (link)].
Guarino A.M., Troiano A., Pizzo E., Bosso A., Vivo M., Pinto G., Amoresano A., Pollice A., La Mantia G, & Calabrò V. (2018). Oxidative Stress Causes Enhanced Secretion of YB-1 Protein that Restrains Proliferation of Receiving Cells. Genes, 9(10), 513.
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2

Cell Proliferation Response to Acetoside

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A total of 6 × 104 HaCaT and A431 cells were seeded in a 12‐well plate; cells were serum‐starved for 24 h; after starvation, total extract or acetoside were added at different concentrations. Every 24 h cells were gently rinsed with 1× PBS, trypsinized, and counted. The count was confirmed by Scepter 2.0 analysis (Millipore, Burlington, MI, USA) as previously described (Fontana, 2018).
Delicato A., Masi M., de Lara F., Rubiales D., Paolillo I., Lucci V., Falco G., Calabrò V, & Evidente A. (2022). In vitro characterization of iridoid and phenylethanoid glycosides from Cistanche phelypaea for nutraceutical and pharmacological applications. Phytotherapy Research, 36(11), 4155-4166.
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