Mouse monoclonal anti stim1 antibody

Rabbit polyclonal anti-STIM1 antibody was from ProSci Inc. (Poway, CA, USA). Mouse monoclonal anti-STIM1 antibody was from BD Biosciences (San Jose, CA, USA). Antibodies against phospho-Ser575-STIM1, phospho-Ser608-STIM1, and phospho-Ser621-STIM1 were produced in collaboration with the Division of Signal Transduction Therapy (DSTT), University of Dundee (Dundee, UK), as described elsewhere^{9 (link), 21 (link), 23 (link)}. Mouse monoclonal anti-ORAI1 (clone G-2) antibody, and anti-ORAI1 (clone G-2) antibody coupled to agarose beads were from Santa Cruz Biotechnology (Heidelberg, Germany). Mouse monoclonal anti-cortactin (clone 4F11) was purchased from Merck Millipore (Darmstadt, Germany). Mouse monoclonal anti-GAPDH antibody was from AbCam (Cambridge, UK). Rabbit anti-GFP antibody was from Cell Signaling Technology (Danvers, MA, USA). All secondary HRP- and Alexa Fluor-labelled antibodies were from ThermoFisher Scientific (Waltham, MA USA).

The rabbit polyclonal anti-STIM1 antibody (raised against the C-terminus) was from ProSci Inc. (Poway, CA, USA), and the mouse monoclonal anti-STIM1 antibody (raised against the N-terminus) was from BD Biosciences (Franklin Lakes, NJ, USA); the mouse monoclonal anti-tubulin beta 3, class III (TUBB3), and the mouse anti-beta tubulin (clone TUB 2.1) were from Sigma-Aldrich (St. Louis, MO, USA); the mouse monoclonal p21 antibody (p21CIP1) and the mouse monoclonal anti-GAPDH antibody were from Santa Cruz Biotechnology (Heidelberg, Germany). Secondary horseradish peroxidase (HRP)-labeled antibodies were from Pierce (Thermo Fisher Scientific, Waltham, MA, USA), or from Santa Cruz Biotechnology (Heidelberg, Germany).