CCl4 and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Formaldehyde (PFA) and bovine serum albumin (BSA) were obtained from Solarbio Technology Co., Ltd. (Beijing, China). iQ™ SYBR Green Supermix was purchased from Bio-Rad (Hercules, CA). Antibody against COLLAGEN (COL1) (ab34710) was purchased from Abcam (MA, USA). Antibodies against FIBRONECTIN (FN) (15613-1-AP) and E-Cadherin (ECAD) (20874-1-AP) were purchased from Proteintech Group, Inc. (Rosemont, USA). Antibody against FXR (SC-25309) was purchased from Santa Cruz Biotechnology (Santa Cruz, USA). Antibodies against alpha-smooth muscle actin (α-SMA) (19245S) and β-ACTIN (4970S) were obtained from Cell Signaling Technology (Danvers, USA).
E cadherin ecad 20874 1 ap
Manufactured by Proteintech
Sourced in United States, United Kingdom
E-Cadherin (ECAD) (20874-1-AP) is a primary antibody that detects the E-Cadherin protein. E-Cadherin is a calcium-dependent cell-cell adhesion glycoprotein that is important for the formation and maintenance of adherens junctions between epithelial cells.
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2 protocols using e cadherin ecad 20874 1 ap
1
Quantitative analysis of extracellular matrix
2
Western Blotting of Cell Markers
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Western blotting was performed according to the standard methods as previously described. The primary antibodies including E-cadherin (E-cad; 20,874-1-AP, 1:50,000), N-cadherin (N-cad; 22,018-1-AP, 1:10,000), SP1 (21,962-1-AP, 1:2500), and GAPDH (10,494-1-AP, 1:40,000) were from Proteintech (Deansgate, Manchester, UK). The secondary antibody goat-anti-rabbit (ab205718, 1:50,000) was from Abcam (Cambridge, UK). GAPDH was the internal control. The densitometry of Western blotting blots was measured using Image J software.
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