Liquid chromatograph

Manufactured by Agilent Technologies

Sourced in United States

A liquid chromatograph is an analytical instrument used to separate, identify, and quantify components in a liquid sample. It functions by passing the sample mixture through a column filled with a stationary phase, which interacts with the sample components, causing them to separate based on their physical and chemical properties.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Vortex genie 2, by Scientific Industries (1 mentions)

Close spelling variants of this product

1200 series High Pressure Liquid Chromatograph 1200 liquid chromatograph Agilent 1100 liquid chromatograph HP 1200L liquid chromatograph 1200 series liquid chromatograph Agilent 1100 series liquid chromatograph 1100 series liquid chromatograph Agilent 1260 series liquid chromatograph HP 1100 series liquid chromatograph Agilent 1290 liquid chromatograph

5 protocols using liquid chromatograph

Gallic Acid Release from Mesoporous Silica

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The release of gallic acid from the mesoporous silica was studied in two simulated biological fluids [31 (link)]: simulated gastric fluid (SGF) with pH = 1.2 and simulated intestinal fluid (SIF) with pH = 6.8, considering the intention to use the materials for oral administration or in the form of suppositories. Release studies were performed at a constant temperature of 37 ± 1 °C and a magnetic stirring of 270 rpm. Working conditions are presented in Table 2.
The amount of active substance released was determined using liquid chromatography using an Agilent liquid chromatograph with DAD-type UV-Vis detector. In order to determine the amount of active substance in the samples, calibration curves were made for gallic acid in the two simulated biological fluids (SGF and SIF) in areas of adequate concentrations relative to the theoretical concentrations of gallic acid.

Petrisor G., Ficai D., Motelica L., Trusca R.D., Bîrcă A.C., Vasile B.S., Voicu G., Oprea O.C., Semenescu A., Ficai A., Popitiu M.I., Fierascu I., Fierascu R.C., Radu E.L., Matei L., Dragu L.D., Pitica I.M., Economescu M, & Bleotu C. (2022). Mesoporous Silica Materials Loaded with Gallic Acid with Antimicrobial Potential. Nanomaterials, 12(10), 1648.

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Metabolite Profiling of Biological Samples

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Agilent Technologies Liquid Chromatograph (Model: 1290 Infinity, United States), Agilent Technologies Quadrupole Time-of-Flight Mass Spectrometer: Agilent Technologies Q-TOF LC/S (Model: 6550 iFunnel), Ultra-low temperature refrigerator (Model: DW-86L 728J, Haier Group), Vortex mixer (Model: Vortex Genie 2, Scientific Industries, United States), table top high speed refrigerated centrifuge (Model: TGL-16M, Shandong Boke Scientific Instrument Co., Ltd., Jinan, China), vacuum centrifugal concentrator (Model: CV200, Beijing Jiaimu Technology Co., Ltd., Beijing, China), automatic snowflake ice maker (Model: IMS-100, Changshu Xueke Electric Appliance Co., Ltd., Changshu, China), and Hisense refrigerator (Model: BCD-206H, Hisense Group Co., Ltd., Beijing, China).
Methanol (Batch Number: 196063, Chromatography pure, Fisher Chemical, Shanghai, China), Acetonitrile (Batch Number: 201643, Chromatography pure, Fisher Chemical, United States), Formic acid (Batch Number: 171662, Chromatographic purity, Fisher Chemical, Shanghai, China), and Wahaha Purified Drinking Water (Hangzhou Wahaha Group Co., Ltd., Shanghai, China).

Cheng C., Zhou M.X., He X., Liu Y., Huang Y., Niu M., Liu Y.X., Gao Y., Lu Y.W., Song X.H., Li H.F., Xiao X.H., Wang J.B, & Ma Z.T. (2022). Metabolomic Analysis Uncovers Lipid and Amino Acid Metabolism Disturbance During the Development of Ascites in Alcoholic Liver Disease. Frontiers in Medicine, 9, 815467.

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Molecular Weight Determination of PLA Composites

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The molecular weights of the neat PLA and reinforced composites were determined by gel permeation chromatography (GPC) in CHCl₃ (2 mg polymer/1 mL solvent) at 30 °C using an Agilent liquid chromatograph equipped with an Agilent degasser, an isocratic HPLC pump (flow rate = 1 mL/min). PS standard was used for the calibration.

Ben Abdeljawad M., Carette X., Argentati C., Martino S., Gonon M.F., Odent J., Morena F., Mincheva R, & Raquez J.M. (2021). Interfacial Compatibilization into PLA/Mg Composites for Improved In Vitro Bioactivity and Stem Cell Adhesion. Molecules, 26(19), 5944.

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GPC Characterization of Polymer Scaffolds

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Gel permeation chromatography (GPC) analyses were performed in CHCl₃ at 30 °C using an Agilent liquid chromatograph equipped with an Agilent degasser, an isocratic HPLC pump (flow rate = 1 mL/min), an Agilent autosampler (loop volume = 100 µL, solution conc. = 1 mg/mL), an Agilent DRI refractive index detector, and three columns: a PL gel 10 µm guard column and two PL gel Mixed-D 10 µm columns (linear columns for separation of MWPS ranging from 500 to 10⁷ g/mol). Polystyrene standards were used for calibration. GPC analyses were performed for the different formulations before/after extrusion and after scaffold production, decontamination, and conditioning. Three samples were tested for each condition.

Bayart M., Dubus M., Charlon S., Kerdjoudj H., Baleine N., Benali S., Raquez J.M, & Soulestin J. (2022). Pellet-Based Fused Filament Fabrication (FFF)-Derived Process for the Development of Polylactic Acid/Hydroxyapatite Scaffolds Dedicated to Bone Regeneration. Materials, 15(16), 5615.

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HPLC Analysis of Bioactive Compounds

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Bioactive
compounds were performed by HPLC on an Agilent Liquid Chromatograph,
equipped with a quaternary pump, an autosampler, a thermostatted column
compartment, and a UV-detector (205 and 296 nm), according to the
method described by Yuan et al. with some modifications.^{26 (link)} The optimized chromatographic column was C18-00G-4375-E0
(250 mm × 4.6 mm; Phenomenex) and mobile phase was 90:10:0.001
(v/v/v) methanol/isopropanol/formic acid at a flow rate of 1 mL/min.
The column was kept at a constant temperature (30 °C), and 10
μL of the sample was injected in all of the analyses. The standard
curve was drawn for each compound and quantified by the external standard
method.

Lv W., Wu C., Lin S., Wang X, & Wang Y. (2021). Integrated Utilization Strategy for Soybean Oil Deodorizer Distillate: Synergically Synthesizing Biodiesel and Recovering Bioactive Compounds by a Combined Enzymatic Process and Molecular Distillation. ACS Omega, 6(13), 9141-9152.

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