Agilent gpc sec software

DMF GPC was used to determine the number-average molecular weight (M_n) and dispersity (Đ) for all homopolymers and diblock copolymers. The instrument set-up comprised two Agilent PL gel 5 μm Mixed-C columns and a guard column connected in series to an Agilent 1260 Infinity GPC system operating at 60 °C. The GPC eluent was HPLC-grade DMF containing 10 mmol LiBr at a flow rate of 1.0 mL min⁻¹, the copolymer concentration was typically 1.0% w/w, and calibration was achieved using a series of ten near-monodisperse poly(methyl methacrylate) standards ranging from 1080 g mol⁻¹ to 905 000 g mol⁻¹. Chromatograms were analyzed using Agilent GPC/SEC software.

¹H and ¹³C NMR were performed on a Bruker DPX‐400 spectrometer, at 400 MHz and 100 MHz, respectively. All chemical shifts were in parts per million (ppm) relative to the tetramethylsilane (TMS) internal standard (0.03% v/v, 0.00 ppm). Coupling constants (J) were expressed in Hertz (Hz). Infrared spectra were recorded on a Bruker ALPHA platinum ATR Fourier transform spectrometer. Absorptions were recorded in wavenumbers (cm⁻¹). Mass spectrometry was achieved using an Agilent 6130B single Quad (ESI). GPC was performed on an Agilent 390‐MDS with autosampler using a PL gel 5.0 µm bead‐size guard column (50 × 7.5 mm), followed by two linear 5.0 µm bead‐size PL gel Mixed D columns (300 × 7.5 mm) and a differential refractive index detector. Using CHCl₃ as eluent the system was calibrated using linear poly(styrene) Easi Vial standards (Agilent Ltd) range from 162 to 5×10⁵ Da. Data were collected and analysed using Cirrus GPC/SEC (v. 3.3) and Agilent GPC/SEC software. TGA, DSC and melting points were carried out using Metler Toledo DSC1‐Star with 40 µL standard aluminium pans and autosampler. Samples heated from −100–70°C, cooled to −100 and heated from −100–600°C under N₂ atmosphere for DSC and heated from 25–600°C for TGA.

Gel permeation chromatography with size-exclusion chromatography (GPC/SEC) analysis was performed on a 1260 Infinity II Multi-Detector GPC/SEC System equipped with an LS, RI, and viscometry detector by Agilent (Santa Clara, CA, USA). The tests were performed using water with 0.02% NaN₃ as the eluent and two PLaquagel-OH Mixed-H 300 × 7.5 mm chromatographic columns (Agilent Technologies, Santa Clara, CA, USA) at a flow rate of 1.0 mL/min. A solution of the sample in water at a concentration of 1 mg ml⁻¹ was prepared. A 50 µL solution was injected into the chromatographic system. Recorded chromatograms were analyzed by Agilent GPC/SEC Software.