Goat anti rabbit igg pre adsorbed

Ultrathin (50 nm) serial sections were cut [using an ultramicrotome (Ultracut E, Reichert-Jung; Vienna, Austria)] and transferred to either carbon-coated (∼80 nm thickness) glass coverslips (13 mm diameter) for scanning EM work, or 200-mesh carbon-coated copper grids (Agar Scientific, Stansted Essex) for transmission EM work. Serial sections were blocked via incubation in TBS/Tween buffer [0.24% Tris (w/v), 0.8% NaCl (w/v) and 0.1% Tween-20 (v/v), pH 8.4] containing 1% BSA (w/v) and 4% normal goat sera (v/v) (Abcam Plc, Cambridge U.K.) for 30 min at room temperature (RT). The TatA protein was labelled by incubation with primary anti-TatA antibody (rabbit monoclonal, as described in [34 (link)]) at a 1:20 dilution (TBS/T + 1% BSA buffer) for 2 h at RT. Sections were then washed with TBS/T + 1% BSA at RT, and then incubated with 10 nm gold-conjugated secondary antibody (Goat anti-rabbit IgG pre-adsorbed, Abcam Plc) for 1 h at RT. Finally, sections were washed in TBS/T (+1% BSA) and dH₂O and allowed to air-dry before insertion into the EM.

Using the touching‐drop method (Rubinstein, 2007), ultrathin sections were blocked via incubation in TBS/Tween buffer (0.24% Tris (w/v), 2.5% NaCl (w/v), and 2% Tween‐20 (v/v)) pH 8.4 containing 1% BSA (w/v) and 4% normal goat sera (v/v) (Abcam Plc, Cambridge, UK) for 30 min at RT. hGH protein was labeled by incubation with primary anti‐hGH antibody (rabbit polyclonal) at 1:20,000 dilution (TBS/Tween + 1% BSA buffer) for 2 hr at RT. Sections were then washed with TBS/Tween + 1% BSA at RT and then incubated with 10 nm gold‐conjugated secondary antibody (Goat antirabbit IgG pre‐adsorbed, Abcam Plc) for 1 hr at RT. Finally, sections were washed in TBS/Tween ( + 1% BSA) and dH₂O and allowed to air dry before insertion into the electron microscope (EM).