Anti actin ab6276

Anti-AMP-activated protein kinase (AMPK; #5831; 1:1000), anti-phospho-AMPK [Thr172] (#2535; 1:1000), anti-ACC (#3676; 1:1000), anti-phospho-ACC [Ser79] (#11818; 1:1000), anti-S6K (#2708; 1:1000), and anti-phopho-S6K [Thr389] (#9234; 1:1000) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-SCD1 (ab39969; 1:500), and anti-actin (ab6276; 1:5000) were purchased from Abcam (Cambridge, MA, USA). Anti-microtubule-associated protein 1A/1B-light chain 3 (LC3; PM036; 1:2000) was purchased from MBL (Nagoya, Japan). Secondary anti-rabbit (#7074; 1:2000) and anti-mouse (715-035-151; 1:10000) antibodies were purchased from Cell Signaling Technology and Jackson ImmunoResearch (West Grove, PA, USA), respectively.

The antibodies included: anti-actin, ab6276 (Abcam, Cambridge, UK); anti-Histone H4 (HIST1H4B), #13919 (Cell Signaling Technology, Danvers, MA, USA); anti- Heat shock cognate 71 kDa protein (HSPA8), #8444 (Cell Signaling Technology); anti-nucleolin (NCL), #12247 (Cell Signaling Technology); anti-nucleophosmin (NPM1), #3542 (Cell Signaling Technology); anti-vimentin (VIM), #3932 (Cell Signaling Technology); anti-plectin-1 (PLEC), #12254 (Cell Signaling Technology). All antibodies were used at the recommended fold dilution described on the manufacture’s datasheet.