Luria bertani broth lb

A total of 88 bacterial strains, including 21 representatives of different species (Table 2) and 67 S. aureus clinical isolates (Table 3) from different clinical materials (wound, pus, furuncle, bronchial tree), of both inpatients (n = 56) and outpatients (n = 11) were used in the study. The isolates originated from the collection of the Department of Medical Microbiology, Medical University of Gdansk and were obtained from 7 clinical centers of Gdansk region between 2013 and 2015. All isolates were identified by conventional bacteriological tests and antimicrobial susceptibility testing was performed by means of the disk diffusion method according to the guidelines of Clinical and Laboratory Standards Institute. All the strains tested were confirmed to be methicillin-resistant. Moreover, the strains were also resistant to erythromycin (96%), clindamycin (91%), norfloxacin (76%) and ciprofloxacin (76%), sulfamethoxazole/trimethoprim (7%), tetracycline (33%), chloramphenicol (27%), gentamicin (10%). All strains assayed were susceptible to fusidic acid, mupirocin and linezolid. Bacteria were stored at −80°C in tryptic soy broth (TSB) supplemented with 10% glycerol. All strains were cultured in Luria-Bertani broth (LB; Biomaxima, Lublin, Poland) or on LB agar plates (LA) solidified with 1.5% (w/v) agar (Becton Dickinson, Franklin Lakes, NJ, USA).