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2 protocols using alexa fluor 594 fab 2 fragment of goat anti rabbit igg h l

1

Immunofluorescence Staining with Alexa Fluor Conjugates

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Alexa Fluor™ 594 donkey anti-goat IgG (H+L), 1:500, Invitrogen A11058; Alexa Fluor™ 594 Fab’2 fragment of goat anti-rabbit IgG (H+L), 1:500, Invitrogen A11072; Alexa Fluor™ 594 Fab’2 fragment of goat anti-mouse IgG (H+L), 1:500, Invitrogen A11020; Alexa Fluor™ 488 goat anti-rabbit IgG (H+L), 1:500, Invitrogen A11008; Alexa Fluor™ 594 goat anti guinea pig IgG (H+L), Invitrogen A1076; Alexa Fluor™ 488 goat anti-mouse IgG (H+L), Invitrogen A11029; Alexa Fluor™ 488 rabbit anti mouse IgG (H+L), 1:500, Invitrogen A11059; Alexa Fluor 488™ Tyramide SuperBoost™ Kit, goat anti-mouse IgG, Invitrogen B40941; Goat anti-mouse IgG (H+L)-HRP conjugate, 1:2000, Bio-Rad 170-6516; Goat anti-rabbit IgG (H+L)-HRP conjugate, 1:2000, Bio-Rad 170-6515
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2

Antibody Protocol for Cell Signaling

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Antibodies against the following proteins were used in this study: GAPDH (Santa Cruz, Dallas, TX, USA), LAMIN B1 (MBL, Tokyo, Japan), PARIS (Millipore), PARIS (Proteintech, Chicago, IL, USA), PGC-1α (Millipore), PDGFRα (R&D, Minneapolis, MN, USA), PPARγ (Santa Cruz) HRP-conjugated F(ab’)2 fragment of goat anti-rabbit IgG (Jackson ImmunoResearch), HRP-conjugated F(ab’)2 fragment of goat anti-mouse IgG (Jackson ImmunoResearch), HRP-conjugated F(ab’)2 fragment of donkey anti-goat IgG (Jackson ImmunoResearch), Alexa Fluor® 594 F(ab’)2 fragment of goat anti-mouse IgG (Invitrogen, Waltham, MA, USA), Alexa Fluor™ 488 F(ab’)2 fragment of goat anti-mouse IgG (H + L) (Invitrogen), and Alexa Fluor™ 594 F(ab’)2 fragment of goat anti-rabbit IgG (H + L) (Invitrogen).
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