Fatty acids were from TCI America (Portland, OR, USA), PEG was from Laysan Bio (Arab, AL, USA), and MTz-NHS was from Click Chemistry Tools. DiI-C18:2, and DiI-C16, DiI-C12 were from ThermoFisher (Waltham, MA, USA). DiI-C18 (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Perchlorate) and DiR (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Iodide) were from Biotium (Hayward, CA, USA). DiI-NH2 was synthesized by a previously reported method.[10 (link)] All the lipids were stored as 1 mM stocks in ethanol. Anticoagulant Citrate Dextrose (ACD) buffer was obtained from the Children’s Hospital Colorado Blood Donation Center and kept sterile before use. Goat IgG fraction to mouse C3 and human C3 (Catalog No. 55 463 and 55 033, respectively) was from MP Biomedicals (Solon, OH, USA). ChromPure human and mouse IgG was from Jackson Immuno Research (West Grove, PA, USA). Secondary antibodies IRDye 80°CW labeled donkey antigoat, goat antihuman, and goat antimouse IgG were from LI-COR Biosciences (Lincoln, NE, USA).
Dii c18 2
Manufactured by Thermo Fisher Scientific
Sourced in United States
DiI-C18:2 is a lipophilic fluorescent dye used for labeling the cell membrane. It contains a long alkyl chain that facilitates its incorporation into the lipid bilayer. DiI-C18:2 emits in the orange-red region of the visible spectrum and is commonly used for cell tracking and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Goat antihuman, by LI COR (2 mentions)
Goat anti mouse igg, by LI COR (2 mentions)
Mtz nhs, by Vector Laboratories (2 mentions)
Diic16, by Thermo Fisher Scientific (2 mentions)
Dii c18, by Biotium (2 mentions)
Goat igg fraction to mouse c3 and human c3 catalog no 55 463, by MP Biomedicals (2 mentions)
Chrompure human and mouse igg, by Jackson ImmunoResearch (2 mentions)
Irdye 80 cw labeled donkey antigoat, by LI COR (2 mentions)
Dii c12, by Thermo Fisher Scientific (2 mentions)
Fatty acids, by Tokyo Chemical Industry (2 mentions)
2 protocols using dii c18 2
1
Lipid Labeling for Cellular Imaging
2
Lipid Labeling for Cellular Imaging
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Fatty acids were from TCI America (Portland, OR, USA), PEG was from Laysan Bio (Arab, AL, USA), and MTz-NHS was from Click Chemistry Tools. DiI-C18:2, and DiI-C16, DiI-C12 were from ThermoFisher (Waltham, MA, USA). DiI-C18 (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Perchlorate) and DiR (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Iodide) were from Biotium (Hayward, CA, USA). DiI-NH2 was synthesized by a previously reported method.[10 (link)] All the lipids were stored as 1 mM stocks in ethanol. Anticoagulant Citrate Dextrose (ACD) buffer was obtained from the Children’s Hospital Colorado Blood Donation Center and kept sterile before use. Goat IgG fraction to mouse C3 and human C3 (Catalog No. 55 463 and 55 033, respectively) was from MP Biomedicals (Solon, OH, USA). ChromPure human and mouse IgG was from Jackson Immuno Research (West Grove, PA, USA). Secondary antibodies IRDye 80°CW labeled donkey antigoat, goat antihuman, and goat antimouse IgG were from LI-COR Biosciences (Lincoln, NE, USA).
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