Dulbecco’s modified Eagle’s medium (DMEM), 0.25% trypsin-EDTA solution and foetal bovine serum (FBS) were purchased from Gibco-BRL (Grand Island, NY, USA). Mouse IL-17A and isotype-control Abs were purchased from R&D Systems (Minneapolis, MN, USA). Monoclonal mouse anti-MMP-2, monoclonal mouse anti-MMP-9 and monoclonal rabbit anti-occludin were purchased from Abcam (Hong Kong, China). Monoclonal mouse anti-claudin-5 antibody was purchased from Invitrogen (Invitrogen, USA). Recombinant IL-17A protein and Fluoroshield mounting medium with 4,6-diamidino-2-phenylindole (DAPI) were purchased from Abcam (Hong Kong, China). The mouse IL-17A ELISA kit, IL-6 ELISA kit and IL-1β ELISA kit were obtained from R&D Systems (Minneapolis, MN, USA).
Mouse il 17a elisa kit
Manufactured by R&D Systems
Sourced in United States
The Mouse IL-17A ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-17A (IL-17A) levels in cell culture supernatants, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β elisa kit, by R&D Systems (1 mentions)
Il 6 elisa kit, by R&D Systems (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Monoclonal rabbit anti occludin, by Abcam (1 mentions)
Anti claudin 5 mouse monoclonal antibody, by Thermo Fisher Scientific (1 mentions)
Fluoroshield mounting medium with 4 6 diamidino 2 phenylindole dapi, by Abcam (1 mentions)
Monoclonal mouse anti mmp9, by Abcam (1 mentions)
3 protocols using mouse il 17a elisa kit
1
Evaluating Inflammatory Cytokine Responses
2
Measuring Mouse IL-17A Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SMC culture supernatants were collected, and the concentrations of IL-17A were measured using the mouse IL-17A ELISA kit (R&D Systems, USA) according to the manufacturer's instructions.
3
Quantifying IL-17A in Lung Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The supernatant of BALF was collected and immediately stored at -70°C until the cytokine assay. IL-17A in lung tissue homogenate and BALF were measured with a mouse IL-17A ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the instructions of the manufacturer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!