Ultrapure water (pH 6.7; Milli-Q, Bedford, MA) was used in all situations throughout the experiments. Sodium tetrachloropalladate (II) (Na2PdCl4), decylamine (DA), 3-Methyladenine (M9281), bafilomycin A1 (B1793), Chloroquine (C6628), MitoTEMPO (SML0737) and VAS2870 (SML0273) were purchased from Sigma-Aldrich Co., LLC. (Shanghai, China). Copper (I) chloride (CuCl), poly(vinyl pyrrolidone) (PVP, Mw≈30000), glucose (α or β form), N,N-dimethylformamide (DMF), and ethanol (C2H6O) were purchased from Sinopharm Chemical Reagent Co. Lid. (Shanghai, China). All chemicals were used as received without further purification. Anti-LC3 antibody (NB100–2220, 1:2000 dilution) were purchased from Novus Biologicals. Anti-GAPDH antibodies (AB9132, 1:10,000 dilution) were from Chemicon.,and anti-ATG5 antibody (sc-133158, 1:500 dilution) were from Santa Cruz Biotechnology. HRP-conjugated anti-rabbit antibody (W4011, 1:10,000 dilution) and HRP-conjugated anti-mouse antibody (W4021, 1:10,000 dilution) were purchased from Promega. The HeLa, 4T1, MCF7 cell lines were purchased from ATCC. MCF-7/MDR (kindly provided by Dr Jun Wang at South China University of Technology) were maintained in the DMEM medium containing doxorubicin.
Anti gapdh antibodies ab9132
Manufactured by Merck Group
Anti-GAPDH antibodies (AB9132) are laboratory reagents used for the detection and quantification of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein in various biological samples. GAPDH is a key enzyme involved in the glycolytic pathway and is commonly used as a loading control or reference protein in Western blot and other protein analysis techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti lc3 antibody nb100 2220, by Novus Biologicals (2 mentions)
Hrp conjugated anti rabbit antibody w4011, by Promega (1 mentions)
Hrp conjugated anti mouse antibody w4021, by Promega (1 mentions)
Lysotracker red dnd 99 l 7528, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Anti sqstm1 sc 28359, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine 3000 l3000015, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti gapdh antibodies ab9132
1
Modulation of Autophagy in Cancer Cells
2
Reagents and Antibodies for Cell Culture
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Regents and antibodies. Cycloheximide (5087390001), MG-132 (474791), wortmannin (W3144), 3methyladenine (M9281), brefeldin A (B5936), CDDP (PHR1624) was purchased from Sigma-Aldrich. LysoTracker Red DND-99 (L7528) and Lipofectamine 3000 (L3000015) were from Invitrogen. Anti-p53 (sc-126, Mouse mAb), anti-ATG5 (sc-133158) and anti-SQSTM1 (sc-28359) antibodies were purchased from Santa Cruz Biotechnology. Anti-ubiquitin (58395S) and anti-K63-polyubiquitin (12930S) antibodies were purchased from Cell Signaling Technology. Anti-LC3 antibody (NB100-2220) was purchased from Novus Biologicals. Anti-GAPDH antibodies (AB9132) was purchased from Chemicon. Goat anti-rabbit (Alexa Fluor 488, A-31566) and goat anti-mouse (Alexa Fluor 647, A-21242) secondary antibodies were from Invitrogen.
Cells. Cell culture reagents were purchased from Gibco (Carlsbad, CA). ES-2 and SK-BR-3 were grown in McCoy's 5A Medium. NCI-H1299 and HUVEC were grown in RPMI 1640 medium. MDA-MB-231, MIA PaCa-2 and HEK 293 were grown in DMEM medium. All mediums were supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) except for MIA PaCa-2, and MIA PaCa-2 were supplemented with 10% fetal bovine serum (Gibco), 2.5% horse serum and 1% penicillin-streptomycin (Gibco). All cell lines were from ATCC and were cultured at 37°C under 5 % CO 2 .
Cells. Cell culture reagents were purchased from Gibco (Carlsbad, CA). ES-2 and SK-BR-3 were grown in McCoy's 5A Medium. NCI-H1299 and HUVEC were grown in RPMI 1640 medium. MDA-MB-231, MIA PaCa-2 and HEK 293 were grown in DMEM medium. All mediums were supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) except for MIA PaCa-2, and MIA PaCa-2 were supplemented with 10% fetal bovine serum (Gibco), 2.5% horse serum and 1% penicillin-streptomycin (Gibco). All cell lines were from ATCC and were cultured at 37°C under 5 % CO 2 .
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