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Sirt7 and foxo1 acetyl lys294

Manufactured by LifeSpan BioSciences
Sourced in United States

Sirt7 and FoxO1 (Acetyl-Lys294) is a lab equipment product offered by LifeSpan BioSciences. It is an antibody that specifically recognizes acetylated lysine 294 of the FoxO1 protein. This antibody can be used for various research applications involving the detection and analysis of acetylated FoxO1 protein.

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3 protocols using sirt7 and foxo1 acetyl lys294

1

Western Blot and Co-immunoprecipitation Analysis of Cellular Signaling

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Tissues and cells were homogenized in Tissue Protein Extraction Reagent or Mammalian Protein Extraction Reagent (#78510 or 78505, Thermo Fisher Scientific, Waltham, MA, USA). Nuclear and cytoplasmic extracts were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (#78835, Thermo Fisher Scientific). Homogenates (20 μg for Western blot and 200 μg for co-IP) were separated by SDS-PAGE and transferred to nitrocellulose membranes. After blocking with 5% skim milk, blots were probed with primary antibodies (Supplementary Table 3) against PAK4, p-PAK4 (S474), HSP90, ubiquitin, HMGCS2, phospho-PKA Substrate, phospho-(Ser/Thr), Sirt1, Sirt6, phospho-Akt (Ser473), Akt, acetylated-Lysine, CHOP, phospho-S6 Ribosomal Protein (Ser240/244), S6 Ribosomal Protein, NCoR1, NEDD4, ATF-6, phospho-PERK (Thr980) (Cell Signaling Technology), PAK4, FGF21, and PPARα (Santa Cruz Biotechnology), Sirt4, lamin B1, and GAPDH (Bioworld Technology, St Louis Park, MN, USA), Sirt5, Sirt7 and FoxO1 (Acetyl-Lys294) (LifeSpan Biosciences, Seattle, WA, USA), and Sirt2, Sirt3, GRP78 BiP, NCOR2/SMRT, CPT1α or CPT1A,T-OXPHOS, THRβ, p-IRE1α and MDM2 (Abcam, Cambridge, UK), LXRα (Proteintech, Rosemont, IL, USA). Immunoreactive bands were detected with an LAS-4000 imager (GE Healthcare Life Science, Pittsburgh, PA, USA).
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2

Western Blot and Co-immunoprecipitation Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues and cells were homogenized in Tissue Protein Extraction Reagent or Mammalian Protein Extraction Reagent (#78510 or 78505, Thermo Fisher Scientific, Waltham, MA, USA). Nuclear and cytoplasmic extracts were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (#78835, Thermo Fisher Scientific). Homogenates (20 μg for Western blot and 200 μg for co-IP) were separated by SDS-PAGE and transferred to nitrocellulose membranes. After blocking with 5% skim milk, blots were probed with primary antibodies (Supplementary Table 3) against PAK4, p-PAK4 (S474), HSP90, ubiquitin, HMGCS2, phospho-PKA Substrate, phospho-(Ser/Thr), Sirt1, Sirt6, phospho-Akt (Ser473), Akt, acetylated-Lysine, CHOP, phospho-S6 Ribosomal Protein (Ser240/244), S6 Ribosomal Protein, NCoR1, NEDD4, ATF-6, phospho-PERK (Thr980) (Cell Signaling Technology), PAK4, FGF21, and PPARα (Santa Cruz Biotechnology), Sirt4, lamin B1, and GAPDH (Bioworld Technology, St Louis Park, MN, USA), Sirt5, Sirt7 and FoxO1 (Acetyl-Lys294) (LifeSpan Biosciences, Seattle, WA, USA), and Sirt2, Sirt3, GRP78 BiP, NCOR2/SMRT, CPT1α or CPT1A,T-OXPHOS, THRβ, p-IRE1α and MDM2 (Abcam, Cambridge, UK), LXRα (Proteintech, Rosemont, IL, USA). Immunoreactive bands were detected with an LAS-4000 imager (GE Healthcare Life Science, Pittsburgh, PA, USA).
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3

Western Blot and Co-immunoprecipitation Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues and cells were homogenized in Tissue Protein Extraction Reagent or Mammalian Protein Extraction Reagent (#78510 or 78505, Thermo Fisher Scientific, Waltham, MA, USA). Nuclear and cytoplasmic extracts were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (#78835, Thermo Fisher Scientific). Homogenates (20 μg for Western blot and 200 μg for co-IP) were separated by SDS-PAGE and transferred to nitrocellulose membranes. After blocking with 5% skim milk, blots were probed with primary antibodies (Supplementary Table 3) against PAK4, p-PAK4 (S474), HSP90, ubiquitin, HMGCS2, phospho-PKA Substrate, phospho-(Ser/Thr), Sirt1, Sirt6, phospho-Akt (Ser473), Akt, acetylated-Lysine, CHOP, phospho-S6 Ribosomal Protein (Ser240/244), S6 Ribosomal Protein, NCoR1, NEDD4, ATF-6, phospho-PERK (Thr980) (Cell Signaling Technology), PAK4, FGF21, and PPARα (Santa Cruz Biotechnology), Sirt4, lamin B1, and GAPDH (Bioworld Technology, St Louis Park, MN, USA), Sirt5, Sirt7 and FoxO1 (Acetyl-Lys294) (LifeSpan Biosciences, Seattle, WA, USA), and Sirt2, Sirt3, GRP78 BiP, NCOR2/SMRT, CPT1α or CPT1A,T-OXPHOS, THRβ, p-IRE1α and MDM2 (Abcam, Cambridge, UK), LXRα (Proteintech, Rosemont, IL, USA). Immunoreactive bands were detected with an LAS-4000 imager (GE Healthcare Life Science, Pittsburgh, PA, USA).
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