Atp standard

Manufactured by Chrono-Log

Sourced in United States

The ATP Standard is a reference material used to calibrate and verify the performance of assays that measure adenosine triphosphate (ATP) levels. It provides a known concentration of ATP for comparison to samples of unknown ATP content.

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Lab products found in correlation

Alexa 488, by Thermo Fisher Scientific (1 mentions) Recombinant human αiibβ3, by R&D Systems (1 mentions) 3 n maleimidylpropionyl biocytin, by Thermo Fisher Scientific (1 mentions) α thrombin, by Merck Group (1 mentions) Chronolume, by Chrono-Log (1 mentions) Abciximab, by Eli Lilly (1 mentions) Human α thrombin, by Merck Group (1 mentions) Luciferin luciferase, by Chrono-Log (1 mentions) Sch 79797, by Cayman Chemical (1 mentions) Adenosine diphosphate (adp), by Chrono-Log (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions)

2 protocols using atp standard

Platelet Activation Assay using Thrombin

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α-thrombin (Sigma); collagen, ATP Standard and CHRONO-LUME (CHRONO-LOG); purified α-thrombin from Dr. J.W. Fenton II was also used [15 (link)]; 3-(N-Maleimidylpropionyl) biocytin (Molecular Probes). Anti-CD41 F(ab)₂ (BD Bioscience) was conjugated with Alexa 488 (Invitrogen). Recombinant human αIIbβ3 (R and D Systems, Catalog Number: 7148-A2).

Wang L., Zhou J., Wang L., Wang C.C, & Essex D.W. (2019). The b′ domain of protein disulfide isomerase cooperates with the a and a′ domains to functionally interact with platelets. Journal of thrombosis and haemostasis : JTH, 17(2), 371-382.

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Purification and Characterization of HuPK and rBbKI

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HuPK was purified as previously described [14 (link)] and the specific activity was found to be 80 U/mg. Recombinant Bauhinia bauhinioides kallikrein inhibitor (rBbKI) was obtained as described [15 (link)]. ADP, ATP-standard, and luciferin-luciferase were from Chrono-Log (Chester, PA, USA). Human α-thrombin (200 NIH units/mg) was from Sigma (St. Louis, MO, USA). The PAR-1 antagonist SCH 79797 was from Cayman Chemical (Ann Arbor, MI, USA); abciximab was from Eli Lilly and Company (Indianapolis, IN, USA), while Fluo-4-AM was from Thermo Fisher Scientific Corporation (Waltham, MA, USA). TLCK was purchased from Merck KGaA /Calbiochem (Darmstadt, Germany).

Ottaiano T.F., Andrade S.S., de Oliveira C., Silva M.C., Buri M.V., Juliano M.A., Girão M.J., Sampaio M.U., Schmaier A.H., Wlodawer A., Maffei F.H, & Oliva M.L. (2017). Plasma Kallikrein enhances platelet aggregation response by subthreshold doses of ADP. Biochimie, 135, 72-81.

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