Log In Sign up
The largest database of trusted experimental protocols

Invitrogen superscript 3 first strand synthesis system for rt pcr

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR is a kit designed for the synthesis of first-strand cDNA from RNA templates. It includes reverse transcriptase, buffer, and other necessary components for the reverse transcription reaction.

Automatically generated - may contain errors

Lab products found in correlation

Allprep dna rna ffpe kit, by Qiagen (2 mentions) Qubit rna hs assay kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

SuperScript III First-Strand Synthesis System for RT-PCR SuperScript III First-Strand Synthesis for RT-PCR SuperScript First-Strand Synthesis System for RT-PCR SuperScript III First-Strand Synthesis System for RT SuperScript III First-Strand System for RT-PCR Invitrogen SuperScript III First-Strand Synthesis System Superscript III RT First-Strand Synthesis system SuperScript First-Strand System for RT-PCR SuperScript III First-Strand Synthesis PCR System Superscript III first strand RT-PCR system

2 protocols using invitrogen superscript 3 first strand synthesis system for rt pcr

1

FFPE RNA Extraction and qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted from a FFPE Raji cell pellet using either the spin-column-based AllPrep® DNA/RNA FFPE kit (Qiagen), according to the manufacturer’s protocol, or a phenol-chloroform-isoamyl alcohol (PCI) extraction procedure.26 (link) Catalyst 3 was incorporated into the AllPrep® protocol by addition of an aqueous solution of 3 at pH 7 to the RNA-containing lysate. The mixture was heated at the stated temperate and for the stated time, then isolation continued as described in the manufacturer’s protocol. RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies). 200 ng RNA was used to synthesize cDNA using the Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies). qPCR was performed in 384-well plates using SYBR® Green dye. Full experimental details for preparation of the FFPE sample, RNA isolation, quantification, cDNA synthesis, and qPCR are provided in the Supporting Data file.
Karmakar S., Harcourt E.M., Hewings D.S., Lovejoy A.F., Kurtz D.M., Ehrenschwender T., Barandun L.J., Roost C., Alizadeh A.A, & Kool E.T. (2015). Organocatalytic Removal of Formaldehyde Adducts from RNA and DNA Bases. Nature chemistry, 7(9), 752-758.
+ Open protocol
+ Expand
2

FFPE RNA Extraction and qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted from a FFPE Raji cell pellet using either the spin-column-based AllPrep® DNA/RNA FFPE kit (Qiagen), according to the manufacturer’s protocol, or a phenol-chloroform-isoamyl alcohol (PCI) extraction procedure.26 (link) Catalyst 3 was incorporated into the AllPrep® protocol by addition of an aqueous solution of 3 at pH 7 to the RNA-containing lysate. The mixture was heated at the stated temperate and for the stated time, then isolation continued as described in the manufacturer’s protocol. RNA was quantified using the Qubit® RNA HS Assay Kit (Life Technologies). 200 ng RNA was used to synthesize cDNA using the Invitrogen SuperScript® III First-Strand Synthesis System for RT-PCR (Life Technologies). qPCR was performed in 384-well plates using SYBR® Green dye. Full experimental details for preparation of the FFPE sample, RNA isolation, quantification, cDNA synthesis, and qPCR are provided in the Supporting Data file.
Karmakar S., Harcourt E.M., Hewings D.S., Lovejoy A.F., Kurtz D.M., Ehrenschwender T., Barandun L.J., Roost C., Alizadeh A.A, & Kool E.T. (2015). Organocatalytic Removal of Formaldehyde Adducts from RNA and DNA Bases. Nature chemistry, 7(9), 752-758.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!