Purelink quick gel extraction pcr purification combo kit

Genomic DNA of the patient was isolated from EDTA-anticoagulated whole blood using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). According to our protocol we developed earlier^{16 (link)}, all exons of the F13A gene (including intron/exon boundaries) were individually amplified by polymerase chain reaction (PCR), followed by agarose gel electrophoresis, and purification of PCR products using the PureLink™ Quick Gel Extraction & PCR Purification Combo Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA). The purified PCR products were sequenced by Microsynth (Balgach, Switzerland) with the same primers as used for PCR. Sequence analysis was performed with NCBI Nucleotide BLAST. For the nomenclature of the mutation, we start numbering of the nucleotides with the ATG start codon, and the numbering of the amino acids with serine, the N-terminal amino acid in the mature FXIII-A protein.