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Fluorodish culture plates

Manufactured by World Precision Instruments
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FluoroDish™ culture plates are a specialized type of cell culture dish designed for fluorescence microscopy applications. They feature a thin, optically clear bottom that allows for high-quality imaging of fluorescently-labeled cells. The plates are made of polystyrene and are available in various sizes to accommodate different experimental requirements.

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Lab products found in correlation

Nis elements analysis software, by Nikon (2 mentions) Ti perfect focus system, by Nikon (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Fluorodish plates FluoroDishes FluoroDish

2 protocols using fluorodish culture plates

1

Live-cell confocal imaging of HEK293T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were captured using a Nikon A1R resonant scanning multispectral confocal microscope (Nikon Instruments, Inc. Melville, NY), equipped with a live cell chamber (37°C with humidified 5% CO2), Nikon Ti Perfect Focus system, and NIS-Elements analysis software (Nikon). 48 h prior to live-cell imaging, HEK293T cells plated onto 0.1% gelatin-coated FluoroDish™ culture plates (35 mm, World Precision Instruments Inc.) were rinsed twice with DMEM (GIBCO) to remove residual lipids and respective treatments were added. Prior to imaging, culture media was refreshed. Time-lapse videos were obtained at 100x magnification (1.45 NA oil objective) at 5–20 s intervals over a 5 min duration.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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2

Live-cell confocal imaging of HEK293T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were captured using a Nikon A1R resonant scanning multispectral confocal microscope (Nikon Instruments, Inc. Melville, NY), equipped with a live cell chamber (37°C with humidified 5% CO2), Nikon Ti Perfect Focus system, and NIS-Elements analysis software (Nikon). 48 h prior to live-cell imaging, HEK293T cells plated onto 0.1% gelatin-coated FluoroDish™ culture plates (35 mm, World Precision Instruments Inc.) were rinsed twice with DMEM (GIBCO) to remove residual lipids and respective treatments were added. Prior to imaging, culture media was refreshed. Time-lapse videos were obtained at 100x magnification (1.45 NA oil objective) at 5–20 s intervals over a 5 min duration.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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