The protein levels of α-SMA, COL1A1, Smad2, p-Smad2, PED7A, PED4A, PKA, p-PKA, total Ras-proximate-1 (Rap1), Guanosine-5′-triphosphate (GTP)-Rap1, and exchange protein directly activated by cAMP 1 (Epac1), cAMP-response element-binding protein (CREB) and p-CREB were examined by immunoblotting following the methods described before (Liu et al., 2018 (link)) using the antibodies listed below: anti-α-SMA (ab5694, Abcam), anti-COL1A1 (ab34710, Abcam), anti-Smad2 (ab40855, Abcam), anti-p-Smad2 (ab53100, Abcam), anti-PDE4A (ab14607, Abcam), anti-PDE4B (ab170939, Abcam), anti-PDE4C (ab170939, Abcam), anti-PDE4D (ab171750, Abcam), anti-PKA (BS-0520R, Woburn, MA, United States), p-PKA (ab75991, Abcam), anti-GTP-Rap1 (ab32373, Abcam), anti-Rap1 (ab14404, Abcam), anti-Epac1 (ab109415, Abcam), anti-CREB (ab31387, Abcam), anti-p-CREB (ab32096, Abcam), and anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ab8245, Abcam) and then with HRP-conjugated secondary antibody. Enhanced chemilumescent (ECL) substrates (Millipore, MA, United States) were used for signals visualization using GAPDH as an endogenous protein for normalization.
Ab14607
Manufactured by Abcam
Sourced in United States
Ab14607 is a primary antibody that recognizes the GAPDH protein. It is suitable for use in Western blotting applications.
Automatically generated - may contain errors
Lab products found in correlation
Ecl substrate, by Merck Group (1 mentions)
Ab31387, by Abcam (1 mentions)
Ab34710, by Abcam (1 mentions)
Ab53100, by Abcam (1 mentions)
Ab32096, by Abcam (1 mentions)
Ab14404, by Abcam (1 mentions)
Ab170939, by Abcam (1 mentions)
Ab32373, by Abcam (1 mentions)
Ab171750, by Abcam (1 mentions)
Ab109415, by Abcam (1 mentions)
Ab75991, by Abcam (1 mentions)
Ab40855, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab5694, by Abcam (1 mentions)
Ab1416, by Abcam (1 mentions)
Ab18203, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
2 protocols using ab14607
1
Protein Expression Analysis of Fibrosis Markers
2
Western Blot Analysis of Cell Signaling Proteins
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Briefly speaking, Western immunoblotting was performed according to the protocol presented previously17 (link). The primary antibodies were rabbit polyclonal to PDE4a (Catalog No.: ab14607, Abcam, USA), mouse monoclonal to E-cadherin (Catalog No.: ab1416, Abcam, USA), rabbit polyclonal to N-cadherin (Catalog No.: ab18203, Abcam, USA), mouse monoclonal to Vimentin (Catalog No.: ab18203, Abcam, USA), and mouse monoclonal to β-actin (Catalog No.: ab8226, Abcam, USA). After incubated with the primary antibodies, the blots were then incubated respectively with secondary antibodies conjugated with HRP (Abcam, USA). Protein levels were detected using the HyGLO HRP detection kit from Denville (Metuchen, NJ, USA). β-actin was used as the internal control.
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