FFPE tissue slides were de-paraffinized and washed with xylene and EtOH. Slides were pretreated, hybridized with FISH probes, and then mounted for microscope observation. Pretreatment was performed using Pretreatment Kit I (02J02-032, Abbott Molecular). Probes were mixed with LSI/WCP Hybridization Buffer (06J67-001, Abbott Molecular). Slides were counterstained with DAPI, and mounted with ProLong® Gold (P36930, Invitrogen). All fluorescence microscopy photos were imaged with NIS-Elements BR2.30. All FISH probes used were from Abbott Molecular: Vysis CEP X (DXZ1) SO Probe (centromeric, 05J08-033), Vysis CEP X (DXZ1) SA Probe (centromeric, 05J09-033), Vysis CEP Y (DYZ1) SGn Probe (q arm, 05J10-034), Vysis CEP Y (DYZ3) SO Probe (centromeric, 05J08-035), and Vysis LSI SRY SO Probe (p arm, 05J27-089).
Wong H.Y., Wang G.M., Croessmann S., Zabransky D.J., Chu D., Garay J.P., Cidado J., Cochran R.L., Beaver J.A., Aggarwal A., Liu M.L., Argani P., Meeker A., Hurley P.J., Lauring J, & Park B.H. (2015). TMSB4Y is a candidate tumor suppressor on the Y chromosome and is deleted in male breast cancer. Oncotarget, 6(42), 44927-44940.
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