Mouse anti alpha tubulin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Mouse anti-alpha-tubulin is a primary antibody that specifically binds to the alpha-tubulin protein, a critical component of the cytoskeleton in cells. This antibody can be used in various laboratory techniques, such as Western blotting, immunofluorescence, and immunohistochemistry, to detect and visualize alpha-tubulin in biological samples.

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Lab products found in correlation

Rabbit anti gapdh 14c10, by Cell Signaling Technology (1 mentions) Rabbit anti gata1, by Cell Signaling Technology (1 mentions) Elyra s 1, by Zeiss (1 mentions) Rabbit anti vwf, by Agilent Technologies (1 mentions) Sample reducing agent, by Thermo Fisher Scientific (1 mentions) Nupage 4 to 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Sample buffer, by Thermo Fisher Scientific (1 mentions) Iblot transfer system, by Thermo Fisher Scientific (1 mentions) Rabbit anti pd l1 e1l3n, by Cell Signaling Technology (1 mentions) Rabbit anti pd1, by Abcam (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Anti gd antibody, by Abcam (1 mentions)

Spelling variants of this product

Anti-Tubulin (26 citations) Alpha-tubulin (12 citations) Mouse anti-tubulin (10 citations) Anti-alpha tubulin (5 citations)

2 protocols using mouse anti alpha tubulin

Platelets GATA1 Enhancer Characterization

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The GATA1 enhancer/HDAC6 deletion was
confirmed by PCR using primers HDAC6-F: 5’-catcttcaagaggatcagagg and
HDAC6-R: 5’-catagctagacactggtt. Electron microscopy of platelets was
performed as described in ref.^{41 (link)}. Immunostaining of resting and fibrinogen spread platelets
was performed as described in ref.^{33 (link)} and analyzed by Structured Illumination Microscopy (SIM,
Elyra S.1, Zeiss, Heidelberg, D.E). Total protein lysates were obtained from
platelets for immunoblot analysis as described in ref.^{55 (link)}. The following antibodies were used for SIM
and immunoblot analysis: rabbit anti-HDAC6 (clone D2E5, Cell Signaling
technology, Danvers, MA, USA), mouse anti-acetylated tubulin antibody (clone
6-11B-1, Sigma, St Louis, MO, USA), mouse anti-alpha-tubulin (A11126, Thermo
Fisher Scientific, Waltham, MA, USA), rabbit anti-VWF (Dako, Agilent
Technologies, Leuven, BE), mouse anti-CD63 and rat anti-GATA1 N6 (Santa Cruz
Biotechnology, Dallas, TX, USA), rabbit anti-GATA1 (NF that was produced against
recombinant N-terminal zinc finger^{56 (link)}), rabbit anti-GAPDH (14C10, Cell Signaling) and
anti-β3 integrin (sc-14009; Santa Cruz Biotechnology). The statistical
analysis of the GATA1 data is described in the Supplementary
Information.

Turro E., Astle W.J., Megy K., Gräf S., Greene D., Shamardina O., Allen H.L., Sanchis-Juan A., Frontini M., Thys C., Stephens J., Mapeta R., Burren O.S., Downes K., Haimel M., Tuna S., Aitman T.J., Bennett D.L., Calleja P., Carss K., Caulfield M.J., Chinnery P.F., Dixon P.H., Gale D.P., James R., Koziell A., Laffan M.A., Levine A.P., Maher E.R., Markus H.S., Morales J., Morrell N.W., Mumford A.D., Ormondroyd E., Rankin S., Rendon A., Richardson S., Roberts I., Roy N.B., Saleem M.A., Smith K.G., Stark H., Tan R.Y., Themistocleous A.C., Thrasher A.J., Watkins H., Webster A.R., Wilkins M.R., Williamson C., Whitworth J., Humphray S., Bentley D.R., Kingston N., Walker N., Bradley J.R., Ashford S., Penkett C.J., Freson K., Stirrups K.E., Raymond F.L, & Ouwehand W.H. (2020). Whole-genome sequencing of patients with rare diseases in a national health system. Nature, 583(7814), 96-102.

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Protein Expression Analysis by Western Blot

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Protein concentrations were equalized in lysis buffer and diluted in lithium dodecyl sulfate Sample Buffer (Invitrogen) and Sample Reducing Agent (Invitrogen). Samples were loaded onto NuPAGE 4 to 12% Bis-Tris Protein Gels (Invitrogen) and transferred onto nitrocellulose using the iBlot Transfer system (Invitrogen). Blots were stained with anti-gD antibody (Abcam), rabbit anti-PD1 (Abcam), rabbit anti-PD-L1 (E1L3N, Cell Signaling), rabbit anti-EPHA3/4/5 (Cell Signaling), rabbit anti-TEM1 (Proteintech), rabbit anti-LRRC15 antibody (abcam), mouse anti-PVR (R&D Systems), mouse anti-Nectin1 (Thermo Fisher), mouse anti-alpha tubulin (Thermo Fisher), and rabbit anti-beta actin (Cell Signaling).

Cao S., Peterson S.M., Müller S., Reichelt M., McRoberts Amador C, & Martinez-Martin N. (2021). A membrane protein display platform for receptor interactome discovery. Proceedings of the National Academy of Sciences of the United States of America, 118(39), e2025451118.

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