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2 protocols using isoorientin

1

Bioactive Lipid Mediator Analysis

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Vasicine, vasicinone, isoorientin, isovitexin, vitexin, apigenin, 12,13-DiHOME, 9,10-DiHOME, 13(S)-HODE, 9(S)-HODE, 13-oxoODE, 9-oxoODE, 12,13-EpOME and 9,10-EpOME, 9-HpODE, and 7-ethoxyresorufin were purchased from Cayman Chemical (Ann Arbor, MI, USA). AITC, ionomycin calcium salt, HC-030039, and A967079 were from Sigma-Aldrich (St. Louis, MO, USA). DMSO was from Fisher Scientific (Waltham, MA, USA).
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2

Quantifying Advanced Glycation Endproducts in Herbal Teas

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Herbal teas samples were purchased from a local market (Taipei, Taiwan). Glyoxal (40 % in aqueous solution), diacetyl (DA), quercetin and o-phenylenediamine were purchased from Alfa Aesar (Great Britain, UK). MethylGlyoxal (40 % in aqueous solution), sodium azide, BSA, glucose, sodium hydroxide, Folin-Ciocalteu reagent, sodium bicarbonate and lanthionine (LAN) were purchased from Sigma (St. Louis, MO, USA). Aspalathin and 3-DG were purchased from Biosynth Carbosynth (St. Gallen, Switzerland). Glucosone, 3-deoxygalactosone (3-DGal), orientin, isoorientin, vitexin, isovitexin, isoquercetin and rutin were purchased from Cayman (Ann Arbor, MI, USA). Nothofagin was purchased from Aobious (Gloucester, MA, USA). CML, CEL, MG-H1, G-H1, methylGlyoxal-lysine dimer (MOLD), Glyoxal-lysine dimer (GOLD) and furosine were purchased form Iris Biotech (Marktredwitz, Germany). Salts, sugar, canola oil, white flour and baking powder were purchased from a local supermarket (Taipei, Taiwan). All solvents used in this study are analytical or LC-MS grade and sourced from Sigma.
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