Sqhax3

Manufactured by Bloomington Drosophila Stock Center

The SqhAX3 is a genetic construct that introduces a specific mutation in the Spaghetti squash (Sqh) gene of Drosophila melanogaster. The Sqh gene encodes the regulatory light chain of non-muscle myosin II, which plays a crucial role in cell division and other cellular processes. The SqhAX3 mutation results in the replacement of a single amino acid in the Sqh protein, leading to altered myosin II function. This construct is a valuable tool for researchers studying the role of myosin II and its regulation in Drosophila development and cellular biology.

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Lab products found in correlation

2 protocols using sqhax3

Drosophila Fly Stocks Maintenance

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Fly stocks were maintained according to standard protocols [46 ]. The following lines were used: E-cadherin-GFP [47 (link)], UAS-GFP, UAS-rpr, UAS-GFP-Moesin, UAS-adoR-RNAi, UAS-mCherry-Moesin, sqh-GFP, and sqh^AX3 (Bloomington Drosophila Stock Center); UAS-kaede (a gift from Wes Grueber [30 (link)]); srp-Gal4 (a gift from Katja Bruckner, University of California, San Francisco [48 (link)]); and UAS-nuclear-red-stinger (a gift from Brian Stramer, King’s College London [26 (link)]).

Weavers H., Liepe J., Sim A., Wood W., Martin P, & Stumpf M.P. (2016). Systems Analysis of the Dynamic Inflammatory Response to Tissue Damage Reveals Spatiotemporal Properties of the Wound Attractant Gradient. Current Biology, 26(15), 1975-1989.

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Drosophila Endocytic Trafficking Regulators

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All crosses and staging were performed at 25°C. Stocks are described in FlyBase (http://flybase.bio.indiana.edu). UAS-YFP-Rab11, UAS-YFP-Rab11^Q70L, UAS-YFP-Rab11^S25N, UAS-Rab11-RNAi, UAS-Cdc42^V12, UAS-YFP-Rab8^Q67L, UAS-YFP-Rab4, UAS-YFP-Rab14^Q97L, UAS-aPKC-RNAi, UAS-Rho1, Dia⁵ FRT40A, UAS-YFP-Rab5, GMR-gal4, Nuf^EY11342, Rho1^72F, Cdc42⁴ FRT19A, UAS-Sec8, Rab11^EP3017, UAS-Rok-CAT, UAS-LIMK-HA, UAS-MBS-RNAi, and Sqh^AX3; RLC-GFP were provided by the Bloomington Stock Center (Indiana University, Bloomington, IN); UAS-Rab5-RNAi was provided by the Vienna Drosophila RNAi Center (Vienna, Austria); UAS-Rok-RNAi and UAS-PKN-RNAi were provided by the National Institute of Genetics (Mishima, Japan); Shg^R69 FRT42D was provided by C. Micchelli (Washington University, St. Louis, MO); Par6^Δ226 FRT19A and UAS-aPKC^CAAX were provided by C. Doe (University of Oregon, Eugene, OR); Sec5^E10 FRT40A and UAS-Sec5 were provided by T. L. Schwarz (Harvard Medical School, Boston, MA); dRip11^d00190 was provided by the Exelixis Collection (Harvard Medical School); and UAS-Rho1 RNAi, UAS-Cdc42 RNAi, and UAS-Cdc42 were generated by our lab (Warner and Longmore, 2009a (link), b (link)).

Yashiro H., Loza A.J., Skeath J.B, & Longmore G.D. (2014). Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II. Molecular Biology of the Cell, 25(19), 2956-2969.

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