Cells were fixed with buffered 4% paraformaldehyde for 10 minutes and stored in ddPBS at 4°C. Cells were permeabilized (0.3% Triton‐X, 2% goat serum in ddPBS) and incubated at 4°C overnight in primary antibodies. Primary antibodies instituted included PDE5A (2395, Cell Signaling) with β2AR (SC‐271322, Santa Cruz) for PDE5A:β2AR PLA, phosphodiesterase 3A (NIH, PDE3A‐CT aa1098‐1115)38 with β2AR (SC‐570, Santa Cruz) for PDE3A:β2AR PLA, and PDE5A (2395, Cell Signaling) with phosphodiesterase 3A (NIH, PDE3A‐CT aa1098‐1115)38 for PDE5:PDE3 PLA. The manufacturer's protocol was followed for proceeding steps. Images were collected on a Zeiss LSM 700 confocal and analyzed on ImageJ by a group‐blinded researcher.
Pde3a
Manufactured by Cell Signaling Technology
PDE3A is a phosphodiesterase enzyme that catalyzes the hydrolysis of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). It plays a role in signal transduction pathways involving these second messengers.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using pde3a
1
Protein-Protein Interaction Imaging
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