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Truseq rna samples preparation kit

Manufactured by Illumina
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Sourced in Australia

The TruSeq RNA Sample Preparation Kit is a laboratory instrument designed for the preparation of RNA samples for sequencing. It provides a streamlined workflow for converting total RNA into a library of template molecules suitable for subsequent cluster generation and sequencing on Illumina platforms.

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Lab products found in correlation

Nanodrop 1000, by Thermo Fisher Scientific (4 mentions) Hiseq 2500 flow cell, by Illumina (2 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions) Rneasy mini kit, by Qiagen (2 mentions)

2 protocols using truseq rna samples preparation kit

1

RNA Extraction and Sequencing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following the 24-h drug treatment, cells were harvested using Trizol, and total RNA was extracted using RNeasy® mini kits (Qiagen, Melbourne, Australia) and quantified by spectrophotometry (NanoDrop 1000 Thermo Fisher Scientific, Waltham, MA, USA). The quality of the extracted RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Melbourne, Australia). RNAseq libraries were prepared from 1ug of total RNA using a TruSeq RNA samples Preparation kit (Illumina, Victoria, Australia). The libraries were sequenced using a HiSeq 2500 flow cell (50 bp single end reads; Illumina) according to the manufacturer’s instructions.
Panizzutti B., Bortolasci C.C., Spolding B., Kidnapillai S., Connor T., Richardson M.F., Truong T.T., Liu Z.S., Gray L., Kim J.H., Dean O.M., Berk M, & Walder K. (2021). Biological Mechanism(s) Underpinning the Association between Antipsychotic Drugs and Weight Gain. Journal of Clinical Medicine, 10(18), 4095.
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2

RNA Extraction and RNAseq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following the 24-h drug treatment, cells were harvested using Trizol, and total RNA was extracted using RNeasy® mini kits (Qiagen, Melbourne, Australia) and quantified by spectrophotometry (NanoDrop 1000 Thermo Fisher Scientific, Waltham, MA, USA). The quality of the extracted RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Melbourne, Australia). RNAseq libraries were prepared from 1 ug of total RNA using a TruSeq RNA samples Preparation kit (Illumina, Victoria, Australia). The libraries were sequenced using a HiSeq 2500 flow cell (50 bp single end reads; Illumina) according to the manufacturer’s instructions.
Panizzutti B., Bortolasci C.C., Spolding B., Kidnapillai S., Connor T., Richardson M.F., Truong T.T., Liu Z.S., Morris G., Gray L., Hyun Kim J., Dean O.M., Berk M, & Walder K. (2021). Transcriptional Modulation of the Hippo Signaling Pathway by Drugs Used to Treat Bipolar Disorder and Schizophrenia. International Journal of Molecular Sciences, 22(13), 7164.
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