8 cpt

Manufactured by Bio-Techne

8-CPT is a synthetic and stable analog of the second messenger cyclic adenosine monophosphate (cAMP). It acts as an activator of protein kinase A (PKA) and can be used in cell-based assays and biochemical studies involving cAMP-dependent signaling pathways.

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Lab products found in correlation

3 protocols using 8 cpt

Neurite Outgrowth Assay with Cerebellar Granule Neurons

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For priming experiments, CGN were treated with 1 mM dbcAMP (Calbiochem; positive control), or with 8-CPT (Tocris) at concentrations of 0.25, 0.5, and 1 mM. Neurons were plated in poly-L-lysine (PLL)-coated 24-well culture dishes at a density of 1×10⁶ neurons per well and incubated for 16 hours. Neurons were then trypsinized, and used in neurite outgrowth assays. Additional CGN were treated with 0.5 or 1 mM 8-CPT, or 1 mM dbcAMP and used immediately in neurite outgrowth assays.

Nikulina E., Gkioka V., Siddiq M.M., Mellado W., Hilaire M., Cain C.R., Hannila S.S, & Filbin M.T. (2020). Myelin-associated glycoprotein inhibits neurite outgrowth through inactivation of the small GTPase Rap1. FEBS letters, 594(9), 1389-1402.

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Neurophysiological Experiment Reagents

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Pharmacological agents were purchased from:

Sigma Aldrich—BAPTA, bicuculline methobromide, TTX, paraformaldehyde, tannic acid, tergitol, d-serine and all the salts used to prepare the internal and external solutions; Tocris Bioscience—(+)-MK-801 maleate, D-AP5, 8-CPT, and CPA. Bio-Rad—glutaraldehyde.

Pérez-Rodríguez M., Arroyo-García L.E., Prius-Mengual J., Andrade-Talavera Y., Armengol J.A., Pérez-Villegas E.M., Duque-Feria P., Flores G, & Rodríguez-Moreno A. (2018). Adenosine Receptor-Mediated Developmental Loss of Spike Timing-Dependent Depression in the Hippocampus. Cerebral Cortex (New York, NY), 29(8), 3266-3281.

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Pharmacological Agents for Neuroscience Research

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Pharmacological agents were purchased from: Sigma Aldrich - BAPTA, d-serine, TTX, sodium fluoracetate, CPA, and all the salts used to prepare the internal and external solutions; Tocris Bioscience - (+)-MK-801 maleate, d-AP5, 8-CPT, cPTIO, l-glutamic acid, LY367385, LY341495, MPEP, 2-AG, AM251, L-NAME, DETA NONOate, Nimodipine, Thapsigargin, THL, GDPβS, Calphostin C, Bicuculline, and SCH50911. These compounds were dissolved in water except 8-CPT, 2-AG, AM251, THL, Nimodipine, and Thapsigargin that were dissolved in dimethyl sulphoxide.

Falcón-Moya R., Pérez-Rodríguez M., Prius-Mengual J., Andrade-Talavera Y., Arroyo-García L.E., Pérez-Artés R., Mateos-Aparicio P., Guerra-Gomes S., Oliveira J.F., Flores G, & Rodríguez-Moreno A. (2020). Astrocyte-mediated switch in spike timing-dependent plasticity during hippocampal development. Nature Communications, 11, 4388.

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