Kms 12 pe

For this study, the MM cell lines IM-9 and RPMI-8226, and the lung carcinoma cell line H226 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The human MM cell line KMS-12-PE was obtained from the Japanese Collection of Research Bioresources (JCRB) (Osaka, Japan). A CHOP^−/− murine embryonic fibroblast (MEF) cell line (CHOP-KO-DR) established from a 13.5-day-old CHOP^−/− mouse embryo by SV-40 immortalization and a CHOP^+/+ MEF cell line (DR-wild-type) established by SV-40 immortalization as a control cell line for CHOP-KO-DR were also obtained from the ATCC. These authorized cell lines were expanded and frozen in aliquots within 1 month after obtaining from the cell banks. Each aliquot was thawed and the cells were used for the experiments within 2 months after thawing. IM-9, H226, RPMI-8226, and KMS-12-PE cells were cultured in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) (Biowest SAS, Nuaillé, France), 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml) (Wako Pure Chemicals Industries, Tokyo, Japan). CHOP-KO-DR and DR-wild-type cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% FBS, 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml). All cell lines were cultured in a humidified incubator containing 5% CO₂ and 95% air at 37°C.