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1 palmitoyl 2 oleoyl 3 linoleoyl rac glycerol

Manufactured by Merck Group
Sourced in United States

1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol is a synthetic lipid compound commonly used in various laboratory applications. It serves as a structural component for the formation of lipid bilayers and membranes, which are essential for the study of cellular and biological processes.

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3 protocols using 1 palmitoyl 2 oleoyl 3 linoleoyl rac glycerol

1

Triglyceride and Lipid Metabolism Analysis

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Glyceryl trilinolein (GTL) and other triglycerides including triolein (GTO), tripalmitin (GTP), and mixed triglycerides 1,2-dilinoleoyl-3-palmitoyl-rac-glycerol (LLP), 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol (LOP), 1,3-dipalmitoyl-2-linoleoylglycerol (PLP), 1,2-dioleoyl-3-palmitoyl-rac-glycerol (OOP), 1,3-dioleoyl-2-palmitoylglycerol (OPO), 1,3-dipalmitoyl-2-oleoylglycerol (POP), linoleic acid (LA), palmitic acid (PA), oleic acid (OA), dimethyl sulfoxide (DMSO), and glycerol reagent were purchased from Sigma-Aldrich (St. Louis, MO). CER (Bachem AG, Bubendorf, Switzerland), IL12 (PeproTech, Rocky Hill, NJ), IL18 (R&D Systems, Minneapolis, MN), PNLIP, IκB-α, cytochrome c antibody (Santa Cruz Biotechnology, Dallas, TX), α-tubulin [Developmental Studies Hybridoma Bank (DSHB), University of Iowa, Iowa], Cox-IV antibody, and TO-PRO-3 iodide (Thermo Fisher Scientific, Waltham, MA) were used.
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2

Comprehensive Lipidomics Analysis Protocol

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Grade MS methanol (MeOH) and 2-propanol, ammonium formate, formic acid, acetic acid, methyl tert-buthyl ether (MTBE) and standards for calibration curves (1,3-dilinoleoyl-rac-glycerol [DG 36:4], 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol [TG 52:3], 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine [LPC 18:1], 1,2-dioleoyl-sn-glycero-3-phosphocholine [PC 36:2], 1,2-dipalmitoyl-sn-glycero-phosphoethanolamine [PE 32:0] and N-palmitoyl-d-erythro-sphingosylphosphorylcholine [SM 34:1]) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Water (milliQ grade) was obtained from a Milli-Q integral water purification system (Millipore Corp., Burlington, MA, USA). For internal standards, SPLASH Lipidomix was purchased from Avanti Polar lipids (Alabaster, AL, USA).
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3

Lipidomics Standards for Plasma Analysis

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Triolein (TG (18:1(9Z)/18:1(9Z)/18:1(9Z)), triα-linolenic acid (TG (18:3(9Z, 12Z, 15Z)/18:3(9Z, 12Z, 15Z)/ 18:3(9Z, 12Z, 15Z))), and 1-Palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol (TG (16:0/18:1(9Z)/18:2(9Z, 12Z))) standards species were purchased from Sigma Aldrich. SPLASH Lipidomix Mass Spectrometry Standard was purchased from Avanti Polar Lipids (Alabaster, AL, US). Pooled human plasma (anticoagulant: Li Heparin) was purchased from Innovative Research (Novi, MI, US). Acetone, methanol, ethyl acetate, acetonitrile, 2-propanol, and ammonium acetate were purchased from Fisher Chemical (Pittsburgh, PA, US). Isooctane and chloroform were purchased from Beijing Tong Guang Fine Chemicals Company (Chaoyang Dist., Beijing, China). A purification system at 0.03 μS cm (model: Micropure UV; Thermo Scientific; San Jose, CA, USA) was used to obtain the deionized water.
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