HPLC was performed using the YMC-Pack Silica or YMC-Pack Pro C18 columns and a Shodex RI-101 detector (Showa Denko K. K., Tokyo, Japan). 1H-NMR spectra were recorded using a Varian Unity 500 (500-MHz) spectrometer (Varian Inc., Palo Alto, CA, USA). Chemical shifts are reported in ppm from tetramethylsilane, used as the internal references (CDCl3: δH 7.26 ppm), with solvent resonance resulting from incomplete deuteration. 13C-NMR spectra were recorded using a Varian Unity 500 (125-MHz) spectrometer with complete proton decoupling (Varian Inc., Palo Alto, CA, USA). Chemical shifts are reported in ppm from tetramethylsilane with the solvent as the internal reference (CDCl3: δC 77.26 ppm). High-resolution mass spectra (HRMS) were obtained with a JEOL JMS-700 spectrometer at the Korea Basic Science Institute (JEOL Ltd., Tokyo, Japan). The optical rotations were measured using a JASCO digital polarimeter (JASCO International Co. Ltd., Tokyo, Japan), using a 5-cm cell. IR spectra were recorded using a JASCO FT/IR-4100 (JASCO International Co. Ltd., Tokyo, Japan). UV spectra were obtained using a Shimadzu UV-1650PC spectrophotometer (Shimadzu Corporation, Kyoto, Japan).
Varian unity 500
Manufactured by Agilent Technologies
Sourced in United States
The Varian Unity 500 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It provides a stable magnetic field and advanced data acquisition capabilities to enable precise chemical analysis and structural characterization of various samples.
Automatically generated - may contain errors
Lab products found in correlation
Tubulin polymerization assay kit, by Cytoskeleton (1 mentions)
Hydrazine hydrate, by Merck Group (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Shodex ri 101 detector, by Resonac (1 mentions)
Uv 1650pc spectrophotometer, by Shimadzu (1 mentions)
Jms 700 spectrometer, by JEOL (1 mentions)
Ft ir 4100, by Jasco (1 mentions)
2 protocols using varian unity 500
1
Analytical Techniques for Compound Characterization
2
Synthesis of Novel Hydrazone Derivatives
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Unless otherwise
mentioned, the reagents and chemicals were purchased from Sigma-Aldrich
(St. Louis, MO) and used without any purification. Aryl hydrazines,
hydrazine hydrate, aryl aldehydes, dimethyl oxalate, and Cu(OTf)2 were purchased from Aldrich and used as received. Tubulin
polymerization assay kit was purchased from Cytoskeleton Inc. and
used in accordance to the instruction manual. α-,β-Unsaturated
ketones20a ,20b and their corresponding hydrazones21 (link) were synthesized according to a reported literature
procedure. Analytical thin-layer chromatography (TLC) was performed
on a Merck silica gel (60 F254) plastic sheet for progress
of the reaction, and column chromatography was performed using Alfa
Aesar 70–200 mesh silica gel. VARIAN UNITY 500 (Varian, Inc.,
Palo Alto, CA) spectrometer operating at 500 MHz equipped with 5 mm
Nalorac QUAD probe was used for recording NMR (1H and 13C) spectra using CDCl3 as a solvent and Me4Si as an internal standard. Chemical shifts (δ) and
spin–spin coupling constant (J) are reported
in ppm and Hz, respectively. Multiplicities are reported as follows:
s = singlet, d = doublet, and m = multiplet. High-resolution mass
spectra (HRMS) were recorded on a quad time-of-flight electrospray
ionization (ESI)-MS instrument.
mentioned, the reagents and chemicals were purchased from Sigma-Aldrich
(St. Louis, MO) and used without any purification. Aryl hydrazines,
hydrazine hydrate, aryl aldehydes, dimethyl oxalate, and Cu(OTf)2 were purchased from Aldrich and used as received. Tubulin
polymerization assay kit was purchased from Cytoskeleton Inc. and
used in accordance to the instruction manual. α-,β-Unsaturated
ketones20a ,20b and their corresponding hydrazones21 (link) were synthesized according to a reported literature
procedure. Analytical thin-layer chromatography (TLC) was performed
on a Merck silica gel (60 F254) plastic sheet for progress
of the reaction, and column chromatography was performed using Alfa
Aesar 70–200 mesh silica gel. VARIAN UNITY 500 (Varian, Inc.,
Palo Alto, CA) spectrometer operating at 500 MHz equipped with 5 mm
Nalorac QUAD probe was used for recording NMR (1H and 13C) spectra using CDCl3 as a solvent and Me4Si as an internal standard. Chemical shifts (δ) and
spin–spin coupling constant (J) are reported
in ppm and Hz, respectively. Multiplicities are reported as follows:
s = singlet, d = doublet, and m = multiplet. High-resolution mass
spectra (HRMS) were recorded on a quad time-of-flight electrospray
ionization (ESI)-MS instrument.
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