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Akta purifier hplc

Manufactured by GE Healthcare
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The Akta Purifier HPLC is a high-performance liquid chromatography (HPLC) system designed for protein purification. It is used to separate, identify, and quantify components in a liquid mixture.

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Lab products found in correlation

Superdex 200 10 30 column, by GE Healthcare (2 mentions) Obitrap fusion mass spectrometer, by Thermo Fisher Scientific (1 mentions) 6520 qtof, by Agilent Technologies (1 mentions) Tecnai 12 transmission electron microscope, by Ametek (1 mentions) Ultrascan 2k ccd camera, by Ametek (1 mentions)

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AKTA purifier (173 citations) AKTA Purifier HPLC system (2 citations)

2 protocols using akta purifier hplc

1

Molecular Characterization of Chemokines

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The molecular sizes of CCL11, varCCL11, CCL4 and variant CCL4 were determined by electrospray ionization mass spectrometry on an Obitrap Fusion mass spectrometer (Thermo Scientific). Size exclusion chromatography was performed on a Superdex 200 10/30 column (GE Healthcare Life Sciences) run at 0.5 mL/min in PBS. UV detection was performed at 214 and 280nm on a GE Akta Purifier HPLC.
Zhang Z., Feng J., Mao A., Le K., La Placa D., Wu X., Longmate J., Marek C., St. Amand R.P., Neuhausen S.L, & Shively J.E. (2018). SNPs in inflammatory genes CCL11, CCL4 and MEFV in a fibromyalgia family study. PLoS ONE, 13(6), e0198625.
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2

Multimodal Characterization of Biomolecules

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Electrospray ionization mass spectrometry (ESI-MS) of small molecules was performed on a Finnigan LTQ or on an Orbitrap Fusion Mass Analyzer (ThermoElectron, San Jose, CA) and molecular weight determination of proteins on a Agilent 6520 QTOF. Electron microscopy was performed on an FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2K CCD camera. LND samples were applied to a glow-discharged 300 mesh Formvar–carbon copper EM grid and stained with 2% uranyl acetate. Size exclusion chromatography (SEC) was performed on a Superdex 200 10/30 column (GE Healthcare Life Sciences) run at 0.5 mL/min in PBS. UV detection was performed at 214 and 280nm on a GE Akta Purifier HPLC. Fluorescence detection was performed on-line on a Jasco Fp-2000 plus set at ex= 470 nm, em= 560 nm.
Wong P., Li L., Chea J., Hu W., Poku E., Ebner T., Bowles N., Wong J.Y., Yazaki P.J., Sligar S, & Shively J.E. (2020). Antibody targeted PET Imaging of 64Cu-DOTA-Anti-CEA PEGylated Lipid Nanodiscs in CEA positive tumors. Bioconjugate chemistry, 31(3), 743-753.
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