Long evans dams

Long-Evans dams, shipped with 10 pups of both sexes that were postnatal day (PD) 22 upon arrival, were obtained from Envigo (Indianapolis, IN). At PD 24, pups were weaned and pair-housed in standard polycarbonate cages. The vivarium was maintained on a reverse 12:12 light-dark cycle with lights off at 09:00. All rats were provided with Teklad 2918 (Envigo) standard chow and water ad libitum, and all experimental procedures were conducted with the approval of the Institutional Animal Care and Use Committee at the Medical University of South Carolina and adhered to the guidelines set forth by the National Research Council’s Guide for the Care and Use of Laboratory Animals (NRC, 2011).

Long-Evans dams were obtained from Envigo (Indianapolis, IN) and shipped with 10 pups of both sexes that were postnatal day (PD) 22 upon arrival. At PD 24, pups were weaned and pair-housed with same sex littermates in standard polycarbonate cages. Rats were maintained on a 12 h/12 h reverse light/dark cycle with ad libitum access to tap water and food (Teklad 2918, Envigo, Indianapolis, IN, USA) and water. All animal procedures were conducted in accordance with the National Institute of Health Guidelines under Institutional Animal Care and Use Committee approved protocols at the Medical University of South Carolina.