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2 protocols using kaempferol 3 o glucoside kf glc

1

Antioxidant Evaluation of Plant Extracts

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6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox); 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH); dimethyl sulfoxide (DMSO), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), acetonitrile, ferric chloride, sulfuric acid, formic acid, hydroxymethylfurfural (HMF), K2HPO3, CaCO3 and sodium hydroxide were purchased from Sigma-Aldrich (Steinheim, Germany). acetonitrile for HPLC was purchased from POCh (Gliwice, Poland). Acetic acid was acquired from Chempur (Piekary Sląskie, Poland). Whereas loganin (Lo), loganic acid (LA), gallic acid (GA), sweroside (S), ellagic acid (EA), p-coumaric acid (p-CuA), 5-O-caffeoylquinic acid (5-CQA, chlorogenic acid), kaempferol 3-O-glucoside (Kf glc), cyanidin 3-O-glucoside (Cy glc) and quercetin 3-O-glucoside (Q glc) were purchased from Extrasynthese (Lyon Nord, France). All reagents were of analytical grade.
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2

Bioactive Compound Extraction and Characterization

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Loganic acid (LA), loganin (L) quercetin 3-O-glucoside (Q-glc), kaempferol 3-O-glucoside (Kf-glc), and cyanidin 3-O-glucoside (Cy-glc) were purchased from Extrasynthese (Genay, France). Further, 2,2-diphenyl-1-picrylhydrazyl (DPPH) ferrous chloride, tripyridyltriazine (TPTZ), potassium persulfate, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), and gallic acid were obtained from Sigma Chemical Co. (Steinheim, Germany). Methanol, acetonitrile, formic acid, and hydrochloric acid were obtained from POCh (Gliwice, Poland). All chemicals and solvents were of analytical grade.
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