Specific pathogen-free (SPF) mice (Shanghai Institutes for Biological Sciences, CSA), TRIzol reagent, DEPC-treated water, SuperScript III reverse transcriptase kit and SYBR quantitative polymerase chain reaction (qPCR) mix (ABI), radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Biotechnology), loading buffer, protease inhibitor and bicinchoninic acid (BCA) protein concentration assay kit (Biosharp), tumor necrosis factor-α (TNF-α), interleukin- (IL-) 6 and myeloperoxidase (MPO) enzyme-linked immunosorbent assay (ELISA) kit (Nanjing Jiancheng Bioengineering Institute), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and secondary antibodies (Boster Biological Technology Co., Ltd.), primary antibodies (Santa Cruz Biotechnology, Inc.), microplate reader (Thermo Fisher Scientific), 7900HT Fast qPCR instrument (Applied Biosystems) and 2500 gel imaging system, tissue homogenizer, and electrophoresis apparatus (Bio-Rad, USA) are used.
7900ht fast qpcr instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 7900HT Fast qPCR instrument is a real-time PCR system designed for quantitative gene expression analysis. It features fast thermal cycling, a compact footprint, and precise temperature control to enable efficient and reliable quantification of nucleic acid samples.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (4 mentions)
Bca protein concentration assay kit, by Biosharp (2 mentions)
Superscript 3 reverse transcriptase kit and sybr quantitative polymerase chain reaction mix, by Thermo Fisher Scientific (2 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Tissue homogenizer and electrophoresis apparatus, by Bio-Rad (1 mentions)
Radio immunoprecipitation assay ripa lysis buffer, by Beyotime (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Elisa kit, by Nanjing Jiancheng (1 mentions)
2 protocols using 7900ht fast qpcr instrument
1
Analyzing Inflammatory Responses in SPF Mice
2
Measuring Inflammatory Cytokine Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Interleukin-6 (IL-6), IL-1 and tumor necrosis factor-α (TNF-α) enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), TRIzol (Invitrogen, Carlsbad, CA, USA) reagent, diethyl pyrocarbonate (DEPC)-treated water, SuperScript III reverse transcriptase kit and SYBR quantitative polymerase chain reaction (qPCR) mix (ABI), radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime Biotechnology, Shanghai, China), loading buffer, protease inhibitor and bicinchoninic acid (BCA) protein concentration assay kit (Biosharp, Heifei, China), β-actin and secondary antibodies (Boster Biological Technology Co., Ltd., Cambridge, MA, USA), primary antibodies (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), tissue homogenizer and electrophoresis apparatus (Bio-Rad, Hercules, CA, USA), microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), 2500 gel imaging system (Bio-Rad, Hercules, CA, USA), and 7900HT Fast qPCR instrument (Applied Biosystems, Waltham, MA, USA).
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