For histological analysis sections of formalin-fixed, paraffin-embedded (FFPE) mouse renal tissue (4 μm) were deparaffinized using xylene followed by graded ethanols for rehydration and stained with hematoxylin and eosin, Periodic-acid schiff (PAS) or Masson’s trichrome according to standard protocol (HistoLab Products AB, Göteborg, Sweden). For IHC boiling in 10 mmol/L citrate buffer at pH 6 was performed as antigen retrieval. Staining was detected using the EnVision system and DAKO Techmate 500 staining equipment according to the instructions of the manufacturer (DAKO, Carpenteria, CA). Paraffin sections were incubated with the following primary antibodies GFP (A290, Abcam, Cambridge, UK), CaIX (AF2344, R&D Systems, Minneapolis, MN), Glut1 (07–1401, Merek Millipore, Darmstadt, Germany), Ki67 (SP6 RM-9601-S, Thermo Scientific, Waltham, MA) and Podocalyxin (AF1556, R&D Systems, Minneapolis, MN). For Oil red O (ORO) stain kidneys were fixed in formalin over night followed by cryo preservation in 30% sucrose over night followed by embedding in optimal cutting temperature compound (O.C.T.) and sectioned (10 μm) on a cryostat.
Podocalyxin af1556
Manufactured by R&D Systems
Podocalyxin (AF1556) is a laboratory reagent produced by R&D Systems. It is a recombinant human Podocalyxin protein.
Automatically generated - may contain errors
2 protocols using podocalyxin af1556
1
Histological Analysis of Mouse Kidney Tissue
2
Immunohistochemical Analysis of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin-fixed and paraffin-embedded lungs were cut into 5-µm sections for Masson's trichrome or hematoxylin and eosin staining. For immunostaining, lung sections underwent antigen retrieval and were stained using antibodies against CD34 (RAM34) (eBiosciences), podocalyxin (AF1556) (R&D Systems), GFP (ab13970) (Abcam), vimentin (ab92547) (Abcam), surfactant protein C (AB3786) (Millipore), E-cadherin (36/E-cad) (BD), and keratin 5 (Poly9059) (Biolegend). Sections were then incubated with AlexaFluor-conjugated antibodies and mounted using Prolong Gold Antifade with DAPI (Life). Optical z-stack images were captured on a Leica SP5X confocal microscope and morphometric analysis was performed using ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!