Log In Sign up
The largest database of trusted experimental protocols

Rat anti cd45

Manufactured by Abcam
Visit Supplier
Sourced in Mongolia, Canada

Rat anti-CD45 is a primary antibody that recognizes the CD45 antigen expressed on the surface of rat leukocytes. CD45 is a transmembrane protein tyrosine phosphatase that plays a critical role in the activation and regulation of T and B cells.

Automatically generated - may contain errors

Lab products found in correlation

Paraformaldehyde (pfa), by BD (1 mentions) Ab16287, by Abcam (1 mentions) A1 confocal microscope, by Nikon (1 mentions) Sudan black b, by Merck Group (1 mentions) Goat anti rat igg, by Abcam (1 mentions) Rabbit anti cd133, by Abcam (1 mentions) Goat anti rabbit igg, by Abcam (1 mentions) Goat anti mouse igg, by Abcam (1 mentions) Inverted fluorescent microscope, by Leica (1 mentions) Mouse anti inos, by BD (1 mentions) Hamster anti podoplanin, by Abcam (1 mentions) Donkey serum, by Merck Group (1 mentions) Goat anti cd20, by Santa Cruz Biotechnology (1 mentions) Rabbit anti vimentin, by Abcam (1 mentions) Rat anti f4 80, by Thermo Fisher Scientific (1 mentions) Rabbit anti cd3, by Agilent Technologies (1 mentions) Donkey anti goat alexa488, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Eclipse ti u microscope, by Nikon (1 mentions)

Close spelling variants of this product

Anti-CD45 (50 citations) Rat anti-human CD45 (3 citations) Rat anti-CD45 antibody (2 citations)

4 protocols using rat anti cd45

1

Immunohistochemical Characterization of Cardiac Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical assessments were carried out on frozen cardiac tissue. Briefly, sections were fixed in 4.2 % paraformaldehyde (BD Biosciences, San Jose CA) for 15 mins, followed by permeabilization and blocking with normal goat serum for 30 minutes at room temperature. Slides were incubated with primary antibodies (Abcam, Cambridge, MA): rabbit anti-CD133 (Catalog # ab19898; used at 1:25), rat anti-CD45 (Catalog # ab30446; used at 1:25), and mouse anti-SSEA-4 (Catalog # ab16287; used at 1:20). The sections were then washed with PBS-Tween, and then incubated with all three secondary antibodies (Abcam, Cambridge, MA) at room temperature for 30 minutes: goat anti-rabbit IgG (Alexa Fluor 488; Catalog # ab150081; used at 1:200), goat anti-rat IgG (Alexa Fluor 647; Catalog # ab150167; used at 1:200), goat anti-mouse IgG (Alexa Fluor 555; Catalog # ab150118; used at 1:200). The sections were finally incubated with 0.1% Sudan Black B (Sigma Aldrich, St. Louis, MO) for 30 minutes. ~20 adjacent areas were imaged at 40x magnification using Nikon Confocal Microscope A1 (Nikon, Tokyo, Japan) in the University of Kentucky Confocal Microscopy facility. Cell numbers were expressed as cells/high power field (HPF). Cell numbers are expressed as cells/HPF.
El-Helw M., Chelvarajan L., Abo-Aly M., Soliman M., Milburn G., Conger A.L., Campbell K., Ratajczak M.Z, & Abdel-Latif A. (2020). Identification of Human Very Small Embryonic Like Stem Cells (VSELS) in human heart tissue among young and old individuals. Stem cell reviews and reports, 16(1), 181-185.
+ Open protocol
+ Expand
2

Fluorescent Imaging of Murine Small Intestine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Small intestinal mesenteries pinned on Sylgard-coated dishes (Dow Corning, Midland, MI) were fixed in 10% formalin for 2 h, washed with Dulbecco's phosphate-buffered saline (DPBS), permeabilized for 2 h with 0.3% Triton X- 100/PBS, and subsequently blocked with 1% bovine serum albumin (BSA) for 2 h. Tissues were then incubated with primary antibody overnight at 4 °C in 0.3% Triton X-100/PBS, followed by secondary antibody for 5 h, and lastly thorough washes with 0.3% Triton X-100/PBS. Primary antibodies were as follows: mouse anti-iNOS (BD Biosciences, San Diego, CA), rabbit anti-n-tyrosine (Bioss, Woburn, MA), hamster anti-podoplanin (Abcam, Cambridge, UK), goat LYVE-1 (R&D, Minneapolis, MN), rat anti-CD45 (Abcam). Tissues were dehydrated in increasing concentrations of ethanol (50, 70, 90, and 100%) for 5 min each, then optically cleared using methyl salicylate for 5–10 min until transparent. Mesenteries were spread onto cover slides and mounted with methyl salicylate. Whole mounts were imaged using an inverted fluorescent microscope (Leica Microsystems, Germany). Each experiment was repeated on 4–5 mice.
Rehal S., Kataru R.P., Hespe G.E., Baik J.E., Park H.J., Ly C., Shin J, & Mehrara B.J. (2020). Regulation of lymphatic function and injury by nitrosative stress in obese mice. Molecular Metabolism, 42, 101081.
+ Open protocol
+ Expand
3

Characterization of Inflammatory Cells in Diabetic Prostate

Check if the same lab product or an alternative is used in the 5 most similar protocols
The number and type of inflammatory cells were characterized in the prostate of Akita and WT mice. Three random areas from each prostatic lobe from each animal were acquired (WT: n = 4; diabetic: n = 4). Neutrophils were identified and quantitated in H&E stained sections. Immunohistochemistry was used to quantitate monocytes/macrophages, T lymphocytes, B lymphocytes, and fibrocytes. Briefly, sections were blocked for 4 hours in PBS containing 10% donkey serum and 1% BSA (both from Sigma-Aldrich, St. Louis, Missouri) was followed by primary antibodies-rat anti-F4/80 (1 : 50, eBioscience, San Diego, CA), rabbit anti-CD3 (1 : 100, Dako, Carpinteria, CA) and goat anti-CD20 (1 : 100, Santa Cruz, Santa Cruz, CA), and rat anti-CD45 (1 : 100, Abcam, Cambridge, MA) and rabbit anti-vimentin (1 : 100, Abcam, Cambridge, MA) overnight at 4 degrees. Secondary antibodies-donkey anti-rat Alexa 594, donkey anti-rabbit Alexa 594, donkey anti-rat Alexa 488, and donkey anti-goat Alexa 488 (1 : 100, Invitrogen, Grand Island, NY) were incubated for one hour at room temperature. Four μg/mL of Hoechst 33258 (Sigma-Aldrich, St. Louis, Missouri) was incubated for 10 minutes. For quantification of each cell type, three random areas from each prostatic lobe from each animal were acquired using Nikon eclipse Ti-U microscope.
Lee S., Yang G., Mulligan W., Gipp J, & Bushman W. (2014). Ventral Prostate Fibrosis in the Akita Mouse Is Associated with Macrophage and Fibrocyte Infiltration. Journal of Diabetes Research, 2014, 939053.
+ Open protocol
+ Expand
4

Immunohistochemical Analysis of Chronic Pain

Check if the same lab product or an alternative is used in the 5 most similar protocols
On 14 and 28 days after induction of EAP based on pelvic tactile allodynia response, mice were anesthetized with 2.5% isoflurane (Isothesia; Butler) and transcardially perfused with cold PBS followed by ice-cold 4% paraformaldehyde. DRGs, spinal cords of cervical, lumbar and sacral segments and prostates were removed and post-fixed overnight in 4% paraformaldehyde. Then the tissues were transferred to 30% sucrose solution overnight. Tissues were mounted with ornithine carbamyl transferase embedding medium (Tissue Tek) on dry ice and stored at −80°C for further experiments. The antibodies used in this study were as follows: rabbit anti-TMEM199 (1:1,000, Abcam), goat anti-IBA1 (1:1,000, Novus), rat anti-CD45 (1:500, Abcam), guinea pig anti-NeuN (1:1,000, Synaptic Systems), rabbit anti-PGP9.5 (1:500, Abcam), mouse anti-CCL2 (1:200, Proteintech) and chicken anti-GFAP (1:1,000, Novus). The multiple immunolabelings were visualized respectively by using Cytm2- or Cytm3-conjugated-donkey anti-rabbit IgG, Cytm3-conjugated-donkey anti-mouse IgG, Cytm3 or 5-conjugated-donkey anti-goat IgG, Cytm3-conjugated-donkey anti-rat IgG, Cytm5-conjugated-donkey anti-Guinea Pig IgG, Brilliant Violet 421-conjugated Donkey Anti-Chicken IgG (1:1,000, Jackson ImmunoResearch). Diamond Antifade mounting medium (Invitrogen) with or without DAPI (dependent on wavelength assignment in multicolor labeling).
Liu Z., Murphy S.F., Wong L., Schaeffer A.J, & Thumbikat P. (2020). Neuronal/astrocytic expression of chemokine (C-C motif) Ligand 2 is associated with monocyte/macrophage recruitment in male chronic pelvic pain. Pain, 161(11), 2581-2591.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!