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Human endothelial progenitor cells

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Human endothelial progenitor cells are primary cells derived from human tissue. They function as precursor cells capable of differentiating into endothelial cells, which are responsible for the formation of blood vessels.

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Lab products found in correlation

Complete growth medium, by Celprogen (3 mentions) Sh sy5y cell, by American Type Culture Collection (3 mentions) Ham s f 12k nutrient mixture, by Corning (3 mentions) Eagle s minimum essential medium, by Corning (3 mentions)

3 protocols using human endothelial progenitor cells

1

Culturing Human Neuroblastoma and Endothelial Progenitor Cells

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham’s F-12 K Nutrient Mixture and Eagle’s Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO2 at 37 °C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12–16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen, Torrance, CA) under standard cell culture conditions (5% CO2, 37 °C) according to the manufacturer’s protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8–11 passages in the present study.
Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H, & Bihl J. (2020). miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway. Stem Cell Research & Therapy, 11, 330.
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2

Culturing Human Cell Lines for Research

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.
Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H., & Chen J. (2020). MiR-137 Boosts the Neuroprotective Effect of Endothelial Progenitor Cell Derived-Exosomes in Oxyhemoglobin Treated SH-SY5Y Cells Partially via COX2/PGE2 Pathway.
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3

In Vitro Cultivation of Neuroblastoma and Endothelial Progenitor Cells

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Cell culture Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.
Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H., & Chen J. (2020). MiR-137 Boosts the Neuroprotective Effect of Endothelial Progenitor Cell Derived-Exosomes in Oxyhemoglobin Treated SH-SY5Y Cells Partially via COX2/PGE2 Pathway.
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