MB49 murine bladder carcinoma cell line (Cat# SCC148) was supplied by EMD Millipore Corporation (Temecula, CA). UMUC-3 and T24 human urinary bladder cancer cell lines (Cat# CRL-1749 and Cat# HTB-4), and Renca murine kidney adenocarcinoma cell line (Cat# CRL-2947), were obtained from ATCC (Manassas, VA). Each cell line was cultured and maintained (no more than 10 passages after initial thawing) according to the company’s instructions. Mycoplasma tests (Lonza, Basel, Switzerland) were performed periodically to ensure that there was no contamination. Both UMUC-3 and Renca cell lines were further transduced with GlowCell 16 FLuc-F2A-GFP lentivirus (Biosettia, San Diego, CA) carrying both firefly luciferase and green fluorescent protein (GFP) genes. Briefly, the cells were seeded in 6-well plates (0.25 × 106 cells/well) and incubated for 3 days with the virus (2×107 IU/well) in the presence of polybrene (10 μg/mL). To ensure more than 95% of cell purity, the cell lines were analyzed and sorted for GFP expression using flow cytometry. The successful transduction was further confirmed by imaging, using an EVOS FL Auto Fluorescence microscope (Life Technology, Carlsbad, CA).
Renca murine kidney adenocarcinoma cell line
Manufactured by American Type Culture Collection
The Renca murine kidney adenocarcinoma cell line is a widely used in vitro model derived from a spontaneous renal adenocarcinoma in a BALB/c mouse. This cell line retains characteristics of the original tumor and is commonly utilized for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Mb49 murine bladder carcinoma cell line, by Merck Group (2 mentions)
Umuc 3 and t24 human urinary bladder cancer cell lines, by American Type Culture Collection (2 mentions)
Mycoplasma test, by Lonza (2 mentions)
Glowcell 16 fluc f2a gfp lentivirus, by Biosettia (2 mentions)
Evos fl auto fluorescence microscope, by Thermo Fisher Scientific (2 mentions)
2 protocols using renca murine kidney adenocarcinoma cell line
1
Transduction of Bladder and Kidney Cancer Cell Lines
2
Cell Line Cultivation and Transduction
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MB49 murine bladder carcinoma cell line (catalog no. SCC148) was supplied by EMD Millipore Corporation. UMUC-3 and T24 human urinary bladder cancer cell lines (catalog nos. CRL-1749 and HTB-4), and Renca murine kidney adenocarcinoma cell line (catalog no. CRL-2947), were obtained from ATCC. Each cell line was cultured and maintained (no more than 10 passages after initial thawing) according to the company's instructions. Mycoplasma tests (Lonza) were performed periodically to ensure that there was no contamination. Both UMUC-3 and Renca cell lines were further transduced with GlowCell 16 FLuc-F2A-GFP lentivirus (Biosettia) carrying both firefly luciferase and GFP genes. Briefly, the cells were seeded in six-well plates (0.25 × 106 cells/well) and incubated for 3 days with the virus (2 × 107 IU/well) in the presence of polybrene (10 μg/mL). To ensure more than 95% of cell purity, the cell lines were analyzed and sorted for GFP expression using flow cytometry. The successful transduction was further confirmed by imaging, using an EVOS FL Auto Fluorescence microscope (Life Technology).
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