Extracts from cortical tissue were prepared using RIPA buffer (Thermo Scientific, Rockford, IL), protease inhibitors (Complete Mini, Roche, Mannheim, Germany) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set III, Calbiochem, San Diego, CA). Samples were homogenized using a Pro Homogenizer (ProScientific Inc., Oxford, CT) and protein concentration was determined using the bicinchoninic acid (BCA) assay (ThermoScientific) with bovine serum albumin as a standard. 30 µg of protein was loaded per lane on a 4–12% gradient acrylamide gel. Proteins were transferred to a nitrocellulose membrane and probed with an anti-phosphorylated ERK (p44/p42 MAPK, #9101, Cell Signaling Technology, Danvers, MA) or an anti-ERK (p44/p42 MAPK, #4695, Cell Signaling Technologies) specific antibody. Western blots were imaged and quantitated using the Licor Odyssey Infrared Imaging System and pERK was normalized to total ERK. Combined data are expressed as the mean ± SEM and Student’s t-test was used to detect significant (p<0.05) differences in the levels of pERK.
Pro homogenizer
Manufactured by PRO Scientific
The Pro Homogenizer is a laboratory equipment designed for the mechanical disruption and homogenization of a wide range of sample types. It utilizes a high-speed rotor-stator mechanism to break down and mix the sample material into a homogeneous suspension. The core function of the Pro Homogenizer is to facilitate the efficient preparation of samples for various analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Complete mini, by Roche (1 mentions)
P44 p42 mapk, by Cell Signaling Technology (1 mentions)
Bca assay, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Bicinchoninic acid bca assay, by Thermo Fisher Scientific (1 mentions)
Sod1 sc 11407, by Santa Cruz Biotechnology (1 mentions)
2 protocols using pro homogenizer
1
Quantification of Phosphorylated ERK Levels
2
Protein Extraction and Western Blot Analysis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!