Sc 14027

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-14027 is a laboratory product offered by Santa Cruz Biotechnology. It is intended for use in scientific research. The core function of this product is to assist researchers in their work, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Millicell ez slide, by Merck Group (1 mentions) Ab6308, by Abcam (1 mentions) Image pro plus 6, by Zeiss (1 mentions) Ab86607, by Abcam (1 mentions) Ab236494, by Abcam (1 mentions) Sc 8432, by Santa Cruz Biotechnology (1 mentions) Rabbit anti phospho erk1 2 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti erk1 2 antibody, by Cell Signaling Technology (1 mentions) Ab32538, by Abcam (1 mentions) Ab59257, by Abcam (1 mentions) Anti β actin, by Sangon (1 mentions) Ab32505, by Abcam (1 mentions) Am2189b, by Abcepta (1 mentions) Rapamycin, by Sangon (1 mentions) Pd98059, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Ly294002, by Merck Group (1 mentions) Ab81283, by Abcam (1 mentions)

3 protocols using sc 14027

Immunofluorescence Imaging of Cardiac Fibroblasts

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Briefly, CFs were seeded in 8-well Millicell EZ slides (Millipore, Germany). After treatment, the CFs were washed with cold phosphate buffered saline (PBS), and then fixed with 4% paraformaldehyde for 20 min at room temperature. After three washes with PBS, the CFs were blocked in 5% goat serum for 1 h and incubated overnight with primary antibodies for Sp1 (1:200, Cat. NO. sc-14027, Santa Cruz, CA, USA) and collagen I (1:500, Cat. NO. ab6308, Abcam, Cambridge) at 4°C. The next day, the CFs were washed with PBS before incubation with secondary antibodies, followed by incubation with DAPI. Images were captured in three blinded sessions by two blinded observers via laser scanning confocal microscopy (LSM710, Carl Zeiss, Germany) and analyzed with Image-Pro Plus 6.0 software.

Li R., Xiao J., Qing X., Xing J., Xia Y., Qi J., Liu X., Zhang S., Sheng X., Zhang X, & Ji X. (2015). Sp1 Mediates a Therapeutic Role of MiR-7a/b in Angiotensin II-Induced Cardiac Fibrosis via Mechanism Involving the TGF-β and MAPKs Pathways in Cardiac Fibroblasts. PLoS ONE, 10(4), e0125513.

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Western Blot Analysis of Cell Signaling Proteins

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The same amount of protein was loaded onto an SDS-PAGE gel, transferred to a nitrocellulose (NC) membrane, and blocked with 50 g/L non-fat milk for 1 h. The membrane was then incubated with the following antibodies at 4°C: rabbit anti-CD147 antibody (MAB972-100, R@D, 1:3,000) and rabbit anti-ERK1/2 antibody (Cell Signaling Technology, 1:5,000), rabbit anti-phospho-ERK1/2 antibody (Cell Signaling Technology, 1:5,000), rabbit anti-Sp1 antibody (sc-14027, Santa Cruz Biotechnology, 1:2,000), rabbit anti-MMP2 antibody (ab86607, abcam, 1:3,000) rabbit anti-MMP9 antibody (ab236494, abcam, 1:2,000), and mouse anti-β-Actin (sc-8432; Santa Cruz Biotechnology,1:5,000). The NC membrane was washed with TBST 3 times for 10 min each time and was then incubated with a secondary antibody at room temperature for 1.5 h, followed by 3 washes in TBST. The membrane was then treated with enhanced chemiluminescence (ECL) reagent. The membrane was scanned and the absorbance (A) values of the protein bands were analysed.

Wang P., Wang Z., Yan Y., Xiao L., Tian W., Qu M., Meng A., Sun F., Li G, & Dong J. (2020). Psychological Stress Up-Regulates CD147 Expression Through Beta-Arrestin1/ERK to Promote Proliferation and Invasiveness of Glioma Cells. Frontiers in Oncology, 10, 571181.

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Cell Culture and Signaling Pathway Modulation

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Cell culture and chemicals. HO-8910, HO-8910pm (a highly metastatic human ovarian cancer) and SKOv3 cell lines were cultured in RPMI-1640 (HyClone, Logan City, UT, USA) medium supplemented with 10% of fetal bovine serum (FBS; gibco, grand Island, NY, USA) and maintained in a humidified incubator at 37˚C and 5% CO 2 . The following antibodies were purchased for western blotting and immunohistochemical staining: anti-AKT (ab32505; Abcam, Cambridge, UK), p70S6K (9202; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-ERK1/2 (AM2189b; Abgent, San Diego, CA, USA), anti-Sp1 (sc14027; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-phospho-AKT (ab81283; Abcam), anti-phsopho-p70S6K (9234; Cell Signaling Technology), anti-phospho-ERK1/2 (ab32538), anti-phospho-Sp1 (T453) and (ab59257) (both from Abcam), anti-phospho-Sp1 (T739) (SAB4504535; Sigma, St. Louis, CA, USA), anti-CD147 (24) , and anti-β-actin (AB10024; Sangon Biotech, Shanghai, China). LY294002 (L9908; Sigma), rapamycin (R706203; Sangon Biotech) and PD98059 (P215; Sigma) were diluted in dimethyl sulfoxide (DMSO).

Zhao J., Ye W., Wu J., Liu L., Yang L., Gao L., Chen B., Zhang F., Yang H, & Li Y. (2015). Sp1-CD147 positive feedback loop promotes the invasion ability of ovarian cancer. Oncology reports, 34(1).

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