MDCK cells (96 well plate format, 5 × 104 cells/well, triplicates) were inoculated with Brisbane/H1N1, pH1N1, NC/H1N1, PR8/H1N1, WI/H1N1, GA/H1N1, and Brisbane/H3N2 at multiplicity of infection (MOI) of 10, or mock infected. At 8 h post-infection (p.i.), cells were fixed with 10% neutral buffered formalin (NBF; Thermo Fisher Scientific, CA, USA) for 1 h and then stained with 1 μg/mL of KPF1-HEK or KPF1-Antx hMAbs, followed by secondary Alexa Fluor 488-conjugated goat anti-human IgG antibody (Life Technologies, Carlsbad, CA, USA). DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific, CA, USA) was used to stain cell nuclei. Fluorescent signal images were acquired under an inverted fluorescent microscope (Olympus, Japan) and analyzed by ImageJ software to measure intensity of the staining (NIH, Bethesda, MD, USA) [53 (link)].
Alexa fluor 488 conjugated goat anti human igg antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 488-conjugated goat anti-human IgG antibody is a secondary antibody that binds to human immunoglobulin G (IgG) antibodies. The antibody is labeled with the Alexa Fluor 488 fluorescent dye, which can be excited at 488 nm and emits green fluorescence. This product is commonly used in immunofluorescence and flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 488 conjugated goat anti human igg antibody
1
Immunofluorescence Assay for Influenza Virus
2
MDCK Cell Influenza Viral Infection Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDCK cells (96 well plate format, 5 x 10 4 cells/well, triplicates) were inoculated with Brisbane/H1N1, pH1N1, NC/H1N1, PR8/H1N1, WI/H1N1, GA/H1N1, and Brisbane/H3N2 at multiplicity of infection (MOI) of 10, or mock infected. At 8 h postinfection (p.i.), cells were fixed with 10% neutral buffered formalin (NBF; Thermo Fisher Scientific, CA, USA) for 1 h and then stained with 1 μg/ml of KPF1-HEK or KPF1-Antx hMAbs, followed by secondary Alexa Fluor 488-conjugated goat anti-human IgG antibody (Life Technologies, Carlsbad, CA, USA). DAPI (4',6-diamidino-2-phenylindole, Thermo Fisher Scientific, CA, USA) was used to stain cell nuclei. Fluorescent signal images were acquired under an inverted fluorescent microscope (Olympus, Japan) and analyzed by ImageJ software to measure intensity of the staining (NIH, Bethesda, MD, USA) [54] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!