Anti cd112

Manufactured by BD

Anti-CD112 is a laboratory reagent used to detect and measure the presence of CD112, a cell surface protein, in biological samples. It is a tool for researchers studying cell-cell interactions and cellular signaling pathways.

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2 protocols using anti cd112

Immunophenotyping of Tumor Cells

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Cell lines were collected by trypsinization (Trypsin-EDTA, Thermo Fischer Scientific) and patient spheroids were digested using TrypLE (Thermo Fischer Scientific) and rested before the staining. Tumor cells were stained with viability cell dye (Zombie NIR, Biolegend) before the staining with anti-CD155 (Clone SKII.4), anti-CD112 (Clone TX31), anti-B7-H6 (Clone # 875001), anti-CD58 (Clone TS2/9), anti-MICA/B (Clone GD4), anti-HLA-ABC (BD Biosciences), anti-PD-L1 (Clone MIH2), anti-HLA class I Bw4 (Clone REA274), anti-HLA-E (Clone 3D12), and anti-EGFR (Clone AY13). Staining of patient derived tumor spheroids was subjected to sample availability.

Marin N.D., Becker-Hapak M., Song W.M., Alayo Q.A., Marsala L., Sonnek N., Berrien-Elliott M.M., Foster M., Foltz J.A., Tran J., Wong P., Cubitt C.C., Pence P., Hwang K., Zhou A.Y., Jacobs M.T., Schappe T., Russler-Germain D.A., Fields R.C., Ciorba M.A, & Fehniger T.A. (2024). Memory-like differentiation enhances NK cell responses against colorectal cancer. Oncoimmunology, 13(1), 2348254.

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NK-cell Phenotyping and Ligand Analysis

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For NK-cell phenotyping, 2.0 X10 5 PBMC were incubated with the appropriated Abs: FITC-conjugated anti-CD3, allophycocyanin-conjugated anti-CD56 and anti-CD69, PE-conjugated anti-NKp30, anti-NKp44, anti-NKp46, anti-CD16, anti DNAM-1, anti-CD94, anti-CD161, anti-CD56, anti-CD158a/h, anti-CD158b, anti-CD107a and anti-CD85j (BD PharMingen) or PE-conjugated anti-NKG2A (R&D Systems), for 30 min at 4°C. The NK-cell population was selected based on CD3 -CD56 + phenotype of gated lymphocytes according to forward-side scatter. For NK-cell ligand analysis, tumor cell lines were incubated with FITC-conjugated anti-HLA-ABC (BD PharMingen), or PE-conjugated anti-HLA-G, anti-HLA-E (Abcam), anti-CD112 and ant-MICA/B (BD PharMingen), anti-CD155 (BioLegend) or respective isotype controls. All samples were acquired on a BD FACS Calibur using Cellquest Pro software (BD Biosciences) and analyzed with FlowJo 7.6.2 software (Tree Star, Inc.).

Roberti M.P., Juliá E.P., Rocca Y.S., Amat M., Bravo A.I., Loza J., Coló F., Loza C.M., Fabiano V., Maino M., Podhorzer A., Fainboim L., Barrio M.M., Mordoh J, & Levy E.M. (2015). Overexpression of CD85j in TNBC patients inhibits Cetuximab-mediated NK-cell ADCC but can be restored with CD85j functional blockade. European journal of immunology, 45(5).

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