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Epiquik dnmt3b activity inhibitor screening assay core kit

Manufactured by Epigentek
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Sourced in United States

The EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit is a lab equipment product that measures the activity or inhibition of DNMT3B, a DNA methyltransferase enzyme. The kit provides the necessary components to perform the assay, including a DNMT3B-specific substrate, detection antibodies, and other required reagents.

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DNMT3B (2 citations) EpiQuikTM (2 citations)

3 protocols using epiquik dnmt3b activity inhibitor screening assay core kit

1

Identifying and Validating miRNA-DNMT3B Interactions

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miRWalk software (http://mirwalk.umm.uni-heidelberg.de/) was used to find the miRNAs related to DNMTs and validate them using Bielefeld Bioinformatics Service software (https://bibiserv.cebitec.uni-bielefeld.de/). The DNMT3B and miRNA-126 binding sites were located. DNMT3B viability was measured using Epigntek’s EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit (Epigentek, Farmingdale, NY, USA).
Cui J., Liu N., Chang Z., Gao Y., Bao M., Xie Y., Xu W., Liu X., Jiang S., Liu Y., Shi R., Xie W., Jia X., Shi J., Ren C., Gong K., Zhang C., Bade R., Shao G, & Ji X. (2020). Exosomal MicroRNA-126 from RIPC Serum Is Involved in Hypoxia Tolerance in SH-SY5Y Cells by Downregulating DNMT3B. Molecular Therapy. Nucleic Acids, 20, 649-660.
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2

DNMT3B Activity Assay in Keratinocytes

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Human keratinocytes cells were treated with 5 µmol Sphin-gosine-1-Phosphate (S–1–P) (Avanti) for 48 hours. Cells were collected and DNMT3B activity was measured using EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit (Epigentek) according to manufacturer's instructions and using recombinant DNMT3B protein, (Active motif) as a positive control. Details in Supplemental Information.
Sinha M., Ghosh N., Wijesinghe D.S., Mathew-Steiner S.S., Das A., Singh K., El Masry M., Khanna S., Inoue H., Yamazaki K., Kawada M., Gordillo G.M., Roy S, & Sen C.K. (2021). Pseudomonas Aeruginosa Theft Biofilm Require Host Lipids of Cutaneous Wound. Annals of Surgery, 277(3), e634-e647.
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3

Quantifying DNMT3B Activity in Keratinocytes

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Human keratinocytes cells were treated with 5µmol S-1-P (Avanti) for 48h. Cells were collected and DNMT3B activity was measured using EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit (Epigentek) according to manufacturer’s instructions and using recombinant DNMT3B protein, (Active motif) as a positive control. Details in Supp inf.
Sinha M., Ghosh N., Wijesinghe D.S., Mathew-Steiner S.S., Das A., Singh K., El Masry M., Khanna S., Inoue H., Yamazaki K., Kawada M., Gordillo G.M., Roy S, & Sen C.K. (2021). Pseudomonas Aeruginosa Theft Biofilm Require Host Lipids of Cutaneous Wound. Annals of Surgery, 277(3), e634-e647.
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