Horseradish peroxidase conjugated goat anti rabbit igg zb 2301

Ovary and kidney samples were homogenized and lysed in lysis buffer (KGP2100, KeyGEN BioTECH, China). Protein extract was collected and subjected to BCA protein assays (Thermo, USA). Thirty microgram of protein was separated by SDS-PAGE and subsequently transferred to PVDF membrane. Afterwards, the membranes were blotted with 10% skim milk for 1h at 37℃ and incubated with primary antibodies against VEGF (WL00009b, Wanleibio, China) and VEGFR1 (AF6204, A nity Biosciences, USA) overnight at 4°C. After incubation with the horseradish peroxidase-conjugated goat anti-rabbit IgG (ZB-2301, ZSGB-BIO, China) for 2h at room temperature, the membranes were visualized with an enhanced chemiluminescence plus kit (P10100, NCM Biotech, China) and Bio-Rad ChemiDoc MP imaging system. Band density was quanti ed using ImageJ software.

Ovary and kidney samples were homogenized and lysed in lysis buffer (KGP2100, KeyGEN BioTECH, China). Protein extract was collected and subjected to BCA protein assays (Thermo, USA). Thirty microgram of protein was separated by SDS-PAGE and subsequently transferred to PVDF membrane. Afterwards, the membranes were blotted with 10% skim milk for 1h at 37℃ and incubated with primary antibodies against VEGF (WL00009b, Wanleibio, China) and VEGFR1 (AF6204, A nity Biosciences, USA) overnight at 4°C. After incubation with the horseradish peroxidase-conjugated goat anti-rabbit IgG (ZB-2301, ZSGB-BIO, China) for 2h at room temperature, the membranes were visualized with an enhanced chemiluminescence plus kit (P10100, NCM Biotech, China) and Bio-Rad ChemiDoc MP imaging system. Band density was quanti ed using ImageJ software.