10-μm coronal cryosections were stained with Nissl staining solution (Beyotime, C0117, Shanghai, China) for 5 min at 37 °C. Then samples were washed using 95% ethyl alcohol for 5 min and dried. Sections were then washed twice in xylene for 5 min. After being sealed with neutral balsam, the slides were observed under an optical microscope (Axio Image A2; Carl Zeiss, Germany) by a blinded investigator.
Axio image a2
Manufactured by Zeiss
Sourced in Germany
The Axio Image A2 is a high-performance optical microscope designed for a variety of applications. It features a modular design and advanced optics to provide precise, high-quality imaging. The core function of the Axio Image A2 is to enable users to observe and analyze samples with exceptional clarity and resolution.
Automatically generated - may contain errors
Lab products found in correlation
Nissl staining solution, by Beyotime (1 mentions)
Anti α smooth muscle actin, by Merck Group (1 mentions)
Ix83 microscope, by Olympus (1 mentions)
Hoechst, by Merck Group (1 mentions)
Cy2 affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Cy5 affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Cellsens dimension software, by Olympus (1 mentions)
4 protocols using axio image a2
1
Nissl Staining of Coronal Cryosections
2
Placental Histopathology and Immunofluorescence
3
Quantifying Histological Lesions in Cardiac Tissue
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An experienced pathologist assessed the histology slides qualitatively on light
microscopy (Zeiss Axio Image A2, Oberkochen, Germany), blind to the groups. At
least twenty cutting areas were randomly chosen and analyzed. The severity of
histological lesions was assessed through parameter-based scores: myocardial
damage, assessed by the presence of contraction bands and eosinophils; leukocyte
infiltration, assessed by the presence of neutrophils, macrophages and
lymphocytes; and interstitial edema. Each parameter was assessed by a score
using the following scale: 0 - absent; 1 - slight; 2 - moderate; 3 - severe; and
4 - very severe[19 (link)].
The total score corresponding to inflammatory lesions was performed by summing
the values ascribed to each parameter for each animal (total ranging from 0 to
12).
microscopy (Zeiss Axio Image A2, Oberkochen, Germany), blind to the groups. At
least twenty cutting areas were randomly chosen and analyzed. The severity of
histological lesions was assessed through parameter-based scores: myocardial
damage, assessed by the presence of contraction bands and eosinophils; leukocyte
infiltration, assessed by the presence of neutrophils, macrophages and
lymphocytes; and interstitial edema. Each parameter was assessed by a score
using the following scale: 0 - absent; 1 - slight; 2 - moderate; 3 - severe; and
4 - very severe[19 (link)].
The total score corresponding to inflammatory lesions was performed by summing
the values ascribed to each parameter for each animal (total ranging from 0 to
12).
4
Quantifying Adipocyte Size in NAFLD
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Random samples of abdominal adipose tissue of rats in the NAFLD and control group were fixed in neutral formalin (10% formaldehyde and 0.1 M phosphate buffer, pH 7) for 24 h at room temperature. The samples were embedded in paraffin, and 7 mm serial sections were then cut using a microtome and stained with hematoxylin and eosin.
Areas were measured in each adipocytes for microscopic field of view, using five fields of view per rat. The average adipocyte area was calculated for each rat, and group means were determined from the individual averages for comparisons between groups. For the above, photomicrographs were obtained at a 40X magnification utilizing an optical microscope (Axio Image A2, Zeiss) with an Olympus digital camera at a 5.1-megapixel resolution. Adipocyte areas were measured using the AxioVision Real 4.6 software (Zeiss Software, Inc.), and expressed as µm 2 .
Areas were measured in each adipocytes for microscopic field of view, using five fields of view per rat. The average adipocyte area was calculated for each rat, and group means were determined from the individual averages for comparisons between groups. For the above, photomicrographs were obtained at a 40X magnification utilizing an optical microscope (Axio Image A2, Zeiss) with an Olympus digital camera at a 5.1-megapixel resolution. Adipocyte areas were measured using the AxioVision Real 4.6 software (Zeiss Software, Inc.), and expressed as µm 2 .
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