Cells were seeded on a microscope slide and washed with phosphate-buffered saline (PBS) after they had adhered to the glass. Goat serum blocking solution was added, followed by the anti-YWHAE (1:100, sc-23957, Santa Cruz Biotechnology, Santa Cruz, CA) and anti-HE4 (1:200, DF8160, Affinity Biosciences, Cincinnati, OH) primary antibody mix. A secondary antibody mixture containing tetramethylrhodamine-labelled goat anti-rabbit IgG (SA00007-2, Proteintech Group Inc., Wuhan, China) and fluorescein isothiocyanate-labelled goat anti-mouse IgG (SA00003-1, Proteintech Group Inc., Wuhan, China) was dropped onto the slide, which was then incubated for 2 h in the dark. Nuclei were stained with 4′,6-diamidino-2-phenylindole (4083S; Cell Signaling Technology Inc., Danvers, MA), and an anti-quenching agent was added dropwise onto the slide immediately before viewing the slices on a confocal microscope.
4 6 diamidino 2 phenylindole 4083s
Manufactured by Cell Signaling Technology
Sourced in United States
4′,6-diamidino-2-phenylindole (4083S) is a fluorescent dye that binds to DNA. It is commonly used in cell biology and molecular biology applications.
Automatically generated - may contain errors
2 protocols using 4 6 diamidino 2 phenylindole 4083s
1
Dual-Labeling Immunofluorescence Assay
2
Dual-Immunofluorescence Staining Protocol
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The cells were seeded on a microscope slide and washed with PBS after they adhered to the glass. Goat serum blocking solution was added, followed by the anti-YWHAE (1:100, sc-23957, Santa Cruz Biotechnology, Santa Cruz, CA) and anti-HE4 (1:200, DF8160, A nity, OH, USA) primary antibody mix. A secondary antibody mixture containing tetramethylrhodamine-labelled goat anti-rabbit IgG (SA00007-2, Proteintech, Wuhan, China) and uorescein isothiocyanate-labelled goat anti-mouse IgG (SA00003-1, Proteintech, Wuhan, China) was dropped onto the slide, which was then incubated for 2 h in the dark. 4′,6diamidino-2-phenylindole (4083S; Cell Signaling Technology, Beverly, MA, USA) was used to stain the cell nucleus, and an anti-quenching agent was added dropwise onto the slide immediately before assessing the slices on a confocal microscope.
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