Bisacrylamide

Bovine serum albumin (BSA), glycine, protease inhibitor cocktail, RA and Tween 20 were from Sigma-Aldrich (St. Louis, MO, USA). CP was procured from Dr Reddy’s Laboratories (Hyderabad, India). Primary and secondary antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and Elabscience (Houston, TX, USA). Eosin, hematoxylin, Ponceau S, β-mercaptoethanol and Triton X-100 were purchased from Hi-Media Laboratories Pvt. Ltd. (Mumbai, India). Acrylamide, bisAcrylamide, ethylene-diaminetetraacetic acid and tris-hydroxymethylaminomethane were purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Horseradish peroxidase was from E. Merck Ltd. (Mumbai, India). Other routine chemicals and reagents were purchased from either Sigma-Aldrich or accredited local vendors.

Penicilliumchrysogenum SNP5 (MTCC13144) strain was locally isolated from grease-contaminated soil of the diesel loco shed and identified by Microbial Type Culture Collection and gene bank Chandigarh, India^{23 (link)}. Triton X-100, Phenylmethylsulfonyl fluoride (PMSF), Lauryl dimethylamine oxide (LDAO), Nicotinamide adenine dinucleotide (NAD) + hydrogen (NADH), 1, 6- diphenyl- 1, 3, 5- hexatriene (DPH), Acrylamide, Bis Acrylamide, Coomassie brilliant blue R-250, Glycine, Bromophenol blue, Methanol, para-nitrophenol, 1-Bromooctane, Dimethyl Sulphoxide (DMSO), Petroleum ether, diethyl ether, other medium components, and Czapek-dox medium were procured from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Hexadecane and THB (Tetrahydrobiopterin), Acetic acid, and Digitonin were procured from TCI Chemicals Pvt. Ltd. (India). Phosphatidylcholine and cholesterol were purchased from Sigma-Aldrich Chemicals Pvt. Ltd. PageRuler™ Plus Prestained Protein Ladder was procured from Thermo Scientific.