Anti ha tag

Manufactured by Fortrea

Sourced in United States

The Anti-HA tag is a lab equipment product used for the detection and purification of proteins that have been engineered to express a specific epitope tag, known as the HA tag. This tag is a short amino acid sequence that can be attached to a protein of interest, allowing for its identification and isolation using antibodies raised against the HA epitope.

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Anti-HA (161 citations) HA-tag (13 citations) Mouse anti-HA tag antibody (2 citations) Mouse monoclonal anti-HA tag antibody (2 citations) Mouse anti–HA-tag (2 citations) Anti-HA tag antibody (2 citations)

3 protocols using anti ha tag

Protein Interactions in Embryonic Stem Cells

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Using the wild-type ESCs, the Flag-tagged CTBP2 expressing ESCs, the Flag-tagged Zfp217 WT rescued ESCs and the Flag-tagged Zfp217 MUT rescued ESCs, immunoprecipitation and western blot were done as previously described (29 (link)). Anti-Zfp217, anti-Zfp516, anti-Zfp518b, anti-Zbtb8b, anti-Hic2, anti-Wiz, anti-Zscan10, anti-Zscan4f antibodies were made by GenScript (Piscataway, New Jersey). Anti-Oct4 (sc-5279) and anti-Znf217 (sc-55351) were acquired from Santa Cruz Biotechnology (Dallas, TX, USA); anti-Nanog (ab14959), anti-Hdac1 (ab7028), anti-Mi-2b (ab72418), anti-Lsd1 (ab17721), anti-H3 (ab1792), anti-H3K4me1 (ab8895), anti-H3K4me3 (ab8580), and anti-H3K27ac (ab4729) were purchased from Abcam (UK); anti-Ctbp1 (612042), anti-Ctbp2 (612044), and anti-Ezh2 (612667) were acquired from BD Transduction Laboratories (San Jose, CA, USA); anti-Esrrb (H6707) and anti-Sox2 (MAB2018) were obtained from R&D System (Minneapolis, MN, USA); anti-Suz12 (3737S), anti-Bax (2772), anti-Caspase-3 (9662S), and anti-Cleaved Caspase-3 (9661S) were from Cell Signaling Technology (Danvers, Massachusetts); anti-CoREST (07-455) and anti-H3K27me3 (07-449) was obtained from Millipore (Germany); anti-HA tag and anti-Myc tag were acquired from Covance (Princeton, NJ, USA); anti-Flag tag and anti-β-Actin were purchased from (Sigma-Aldrich, St. Louis, MO, USA).

Kwak S., Kim T.W., Kang B.H., Kim J.H., Lee J.S., Lee H.T., Hwang I.Y., Shin J., Lee J.H., Cho E.J, & Youn H.D. (2018). Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation. Nucleic Acids Research, 46(13), 6592-6607.

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Western Blot Analysis of Luciferase Assays

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Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% non-fat milk at room temperature for 15 min for blocking and then incubated with the primary antibody at 4°C overnight. After washing the membrane with TBST three times, the membrane was then incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz) at RT for 1 h. The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3,000 for anti-β-actin (Millipore-Sigma) and K-Rta (76 (link)).

Izumiya Y., Algalil A., Espera J.M., Miura H., Izumiya C., Inagaki T, & Kumar A. (2024). Kaposi’s sarcoma-associated herpesvirus terminal repeat regulates inducible lytic gene promoters. Journal of Virology, 98(2), e01386-23.

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Investigating SRF Regulation via GSK3β

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Anti-HA-tag (Covance, D14EF01045), anti-GFP-tag (Proteintech, 66002-1-1g), anti-SRF, anti-β-Actin (Santa Cruz, sc-47778), anti-p-GSK3β (Cell Signaling technology, 9336). cycloheximide (CHX), MG132, lactacystin, 3-methyladenine (3-MA), and NH₄Cl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Constitutively active GSK3β (GSK3β-CA) was obtained from Addgene, HA-SRF plasmid was obtained from Addgene, GFP-SRF plasmid was obtained from Addgene.

Luo J., Jin F.Q., Yin M, & Jin Z.G. (2019). Regulation of SRF protein stability by an autophagy-dependent pathway. Biochemical and biophysical research communications, 521(2), 279-284.

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