C57bl 6j

Male C57BL/6J and ICR mice were obtained from Japan SLC, Inc. Juvenile, male C57BL/6J mice (3 weeks old) were used to exposure to social defeat stress. Aggressive, male ICR mice (over 7 weeks old) for social defeat stress were screened based on the duration of attacks on C57BL/6J mice, with more than 1/10 minutes as the inclusion criterion. Unfamiliar target male ICR mice (over 4–6 weeks old) were used for the social interaction test. They were housed in plastic cages in a regulated environment (23°C ±1°C, 50±5% humidity), with a 12-hour-light/-dark cycle (lights on at 9:00 am). Food (CE2; Clea Japan Inc.) and tap water were available ad libitum. All experiments were conducted in accordance with the Guidelines for Animal Experiments of the Nagoya University Graduate School of Medicine. Procedures involving animals and their care were conformed to the international guidelines set out in the National Institutes of Health’s Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 1978).

ICR and C57BL/6J mice were purchased from Japan SLC (Hamamatsu, Japan). They were bred under the controlled condition (temperature 22 ± 2°C, humidity 50 ± 10%, and 12–12 hr light-dark
cycle) with water and food ad libitum. After 1-week acclimation, eight to ten-week-old virgin mice were paired in syngeneic in the evening. On the next morning, the mice
confirmed vaginal plugs were designated as day 0.5 of pregnancy (D0.5).
ICR mice were euthanized by cervical dislocation after deep anesthesia by isoflurane. More than three pregnant mice were used at each time point and more than five pregnant implantation
sites were collected from each pregnant mouse. Specimens were fixed in 10% neutral-buffered formalin for histological studies. Other samples were stored by rapid freezing for quantitative
PCR and western blot.
All mice used in this study were kept under veterinary health control and received care along with the guideline for the proper implementation of animal use at the Yamaguchi University.
Experimental designs were passed preliminary reviews by the Ethical Committee on Animal Experimentation at Yamaguchi University (Approval numbers: 361 and 443).

Female C57BL/6J and SCID mice aged 6 weeks were purchased from Japan SLC (Hamamatsu, Japan) and CLEA Japan (Tokyo, Japan), respectively. The mice were maintained under specific pathogen-free conditions. All mouse experiments were carried out in compliance with the Guidelines for Animal Experimentation from Shiga University of Medical Science (Shiga, Japan).
The mouse lymphoma cell line E.G7 that expresses ovalbumin (OVA) and the natural killer (NK) cell-sensitive cell line YAC-1 were purchased from ATCC (Manassas, VA, USA), and were passaged for fewer than 6 months. The mouse Lewis lung carcinoma cell line LLC1 and the mouse melanoma cell line B16F1 were provided by the Cell Resource Center for Biomedical Research, Tohoku University (Sendai, Japan).
Tranilast (N-[3,4-dimethoxycinnamoyl]-anthranilic acid) (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in DMSO at a concentration of 25 mM as a stock solution.