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2 protocols using ammonium hydroxide

1

Synthesis and Characterization of Iron Oxide Nanocomposites

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Iron(iii) chloride hexahydrate (FeCl3·6H2O), iron(ii) chloride tetrahydrate (FeCl2·4H2O), graphite nanopowder (type-1), paraffin liquid oil (extrapure), potassium permanganate (KMnO4), ammonium hydroxide (NH4OH), concentrated sulphuric acid (H2SO4), hydrogen peroxide (H2O2), sodium nitrate (NaNO3), potassium chloride (KCl), potassium ferricyanide ([K3Fe(CN)6]) extrapure 98%, agarose (low EEO), CaCl2 fused pure (90–95%), sucrose, sodium borohydride powder (NaBH4), magnesium sulphate dried extrapure (MgSO4·xH2O), sodium phosphate monobasic dihydrate extrapure (NaH2PO4·2H2O) and sodium bicarbonate extrapure (NaHCO3) were purchased from SRL chemicals. Dopamine hydrochloride, uric acid, acetic acid and serotonin hydrochloride were purchased from Sigma-Aldrich. Chitosan (0.5% in 0.5% acetic acid) obtained from Tokyo Chemical Industry (TCI Chemicals). Glucose (d-glucose anhydrous) was purchased from Nice Chemicals. All the mentioned chemicals were used for analysis without any further purifications.
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2

Cerium-Vanadium Nanocomposite Enzyme Assay

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Cerium (III) nitrate hexahydrate (Ce (NO3)3·6H2O), ammonium metavanadate
(NH4VO3), ammonium hydroxide (NH4OH), lithium hydroxide (LiOH), sodium chloride, sodium acetate, acetic
acid, potassium chloride, uric acid, glutathione reduced (GSH), and
lysine were purchased from SRL chemicals, India, and used without
further purification. Native enzyme peroxidase from horseradish, hydrogen
peroxide (30%), 3,3,5,5′-tetramethylbenzidine (TMB), sodium
acetate, l-cysteine, ascorbic acid, urea, glucose, 4-nitrophenol,
and phosphate-buffered saline (PBS) tablets were obtained from Sigma-Aldrich.
Ultrapure Milli-Q water was used for all the experiments and preparation
of buffers.
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