RT4, T24, SVHUC1, UMUC3 cells were obtained from American Type Culture Collection (ATCC). SVHUC1(transformed, SV40 immortalized) cells were cultivated in Ham's F12K (ATCC 30-2004, Manassas, VA. USA) media supplemented with 7% fetal bovine serum (FBS) (S11195, Atlanta Biologicals, Lawrenceville, GA. USA) and 1% penicillin-streptomycin (30-002-Cl) (Corning Cellgro®, Manassas, VA. USA). Bladder cancer cell lines, T24, and RT4 were cultured in McCoy's 5A (Corning), FBS (10%) and antibiotics. UMUC3 were cultured in MEM media (Corning), FBS (10%), sodium pyruvate (1%), non-essential amino acids (0.1%), sodium bicarbonate (2%). The human bladder epithelial cell lines HBLEC (FC-0040 Lot # 00997 & 01177) was purchased from Lifeline Cell Technology (Oceanside, CA) and were cultivated in ProstaLife™ basal media (LM-0017) supplemented with the ProstaLife™ LifeFactors kit (LS-1072) from LifeLine Cell Technology.
Ham s f 12k
Manufactured by American Type Culture Collection
Sourced in United States
Ham's F-12K is a cell culture medium formulated for the growth and maintenance of various mammalian cell lines. It provides the essential nutrients and growth factors required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Um uc 3, by Corning (1 mentions)
Mem media, by Corning (1 mentions)
Umuc3, by American Type Culture Collection (1 mentions)
Sv huc 1, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Mccoy s 5a, by Corning (1 mentions)
Dmem media, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
H1975, by American Type Culture Collection (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Endothelial cell medium (ecm), by Thermo Fisher Scientific (1 mentions)
Huvecs, by Thermo Fisher Scientific (1 mentions)
Huvecs, by ScienCell (1 mentions)
Amphotericin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Human a549 cells, by American Type Culture Collection (1 mentions)
5 protocols using ham s f 12k
1
Bladder Cell Line Cultivation Protocol
2
Cell Culture Conditions for HEK293T, JAR, and BeWo
3
Cell Culture of Lung Cancer Cell Lines
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Cell lines HCC827 and H1975 (American Type Culture Collection, Manassas, VA) were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin–streptomycin. Cell line A549 (American Type Culture Collection, Manassas, VA) was cultured in Ham’s F-12K (Kaighn's) medium supplemented with 10% FBS and 100 U/mL penicillin–streptomycin. Cells were maintained under standard cell culture conditions at 37 °C and 5% CO2 in humid conditions.
4
Cytotoxicity of Isomangiferin on Breast Cancer Cell Lines
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Human breast carcinoma cell lines used for in vitro experiments included MDA-MB-231, T47D, MCF7, and SKBR3. The 4T1 triple-negative mouse breast cancer cell line was also used. The MCF-10A immortalized normal human mammary epithelial cell line was used as a normal control to test the cancer cell cytotoxicity of isomangiferin. All cells were purchased from the American Type Culture Collection (Manassas, USA) and cultured in high-glucose Dulbecco's modified Eagle's medium or Ham's F12K (Kaighn's) medium containing 10% FBS in an atmosphere of 5% CO2 at 37℃. Primary human umbilical vein endothelial cells (HUVECs; ScienCell Research Laboratories, San Diego, USA) were used as a model to mimic the process of angiogenesis. The HUVECs were cultured in Endothelial Cell Medium (Gibco Life Technology) supplemented with 5% FBS, 1% endothelial cell growth supplement, and 1% penicillin-streptomycin in an atmosphere of 5% CO2. The culture medium was refreshed every 2 to 3 days.
5
Isolation and Culture of Alveolar Epithelial Cells
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Sprague-Dawley rat AEC type 1 (AT1) cells were isolated by immunoselection for T1-alpha by methods we have previously published25 (link). Resultant AT1 were seeded on glass coverslips, cultured in high glucose Dulbecco’s Modified Eagle Media (DMEM) supplemented with 10% FBS, penicillin, streptomycin, and amphotericin (100 U/ml, 100 µg/ml, and 25 µg/ml, respectively; Sigma, St. Louis, MO), and maintained in a 5% CO2 humidified 37 C incubator until day of the experiment. All AT1 experiments were performed on day 5 post-harvest.
Human A549 cells (American Type Culture Collection, Manassas, VA) were cultured in Ham’s F-12K containing L-glutamine (2 mM), and FBS and penicillin-streptomycin-amphotericin B as described for AT1. A549 cells were passaged 48 h prior to each experiment, resulting in 85–90% confluent monolayers.
Human A549 cells (American Type Culture Collection, Manassas, VA) were cultured in Ham’s F-12K containing L-glutamine (2 mM), and FBS and penicillin-streptomycin-amphotericin B as described for AT1. A549 cells were passaged 48 h prior to each experiment, resulting in 85–90% confluent monolayers.
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