The monoisotopic molecular mass and purity of native cathelicidin-OA1 was determined using an AXIMA-CFRTM plus MALDI-TOF mass spectrometer (Shimadzu/Kratos, Manchester, UK) in linear mode with α-cyano-4-hydrorycinnamic acid as the matrix. All procedures were carried out per the manufacturer’s standard protocols, and data were analyzed using the provided software package. To obtain the complete amino acid sequence, the peptide was subjected to Edman degradation on a PPSQ-31A protein sequencer (Shimadzu, Japan) according to the manufacturer’s standard GFD protocols, and recognition of cysteine residues was conducted according to the procedures provided by the manufacturer.
Axima cfrtm plus maldi tof mass spectrometer
Manufactured by Shimadzu
Sourced in United Kingdom, Jamaica
The AXIMA-CFRTM plus MALDI-TOF mass spectrometer is a laboratory equipment designed for high-performance molecular analysis. It utilizes Matrix-Assisted Laser Desorption/Ionization (MALDI) technology coupled with Time-of-Flight (TOF) mass spectrometry to accurately determine the molecular masses of a wide range of analytes, including proteins, peptides, oligonucleotides, and small molecules.
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Lab products found in correlation
2 protocols using axima cfrtm plus maldi tof mass spectrometer
1
Mass Spectrometric Analysis of Cathelicidin-OA1
2
Determining Protein Molecular Mass and Sequence
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The average molecular mass of native OA-GL21 was determined by an AXIMA-CFRTM plus MALDI-TOF mass spectrometer (Shimadzu/Kratos, Manchester, U.K.) in linear mode with α-cyano-4-hydrorycinnamic acid as the matrix. Then the high-purity peptide was directly subjected to Edman degradation on a PPSQ-31A protein sequencer (Shimadzu, Japan) to obtain the complete amino acid sequence according to the manufacturer’s standard GFD protocols provided by the manufacturer, and data were analyzed using the provided software package.
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